Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
Journal article, 2014

Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.

cell proliferation

osteogenic differentiation

colloidal lithography

implantable materials

human mesenchymal stem cells

nanotopography

mineralization

Author

Giuseppe Maria de Peppo

University of Gothenburg

Hossein Agheli

University of Gothenburg

Camilla Karlsson

University of Gothenburg

Karin Ekström

University of Gothenburg

H. Brisby

University of Gothenburg

Maria Lennerås

University of Gothenburg

Stefan Gustafsson

SuMo Biomaterials

Chalmers, Applied Physics, Eva Olsson Group

Peter Sjövall

Chalmers, Applied Physics, Biological Physics

Anna Johansson

University of Gothenburg

Eva Olsson

Chalmers, Applied Physics, Eva Olsson Group

Jukka Lausmaa

SP Sveriges Tekniska Forskningsinstitut AB

BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy

Peter Thomsen

University of Gothenburg

Sarunas Petronis

SP Sveriges Tekniska Forskningsinstitut AB

BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy

International journal of nanomedicine

1176-9114 (ISSN) 1178-2013 (eISSN)

Vol. 9 1 2499-2515

Subject Categories

Biomaterials Science

DOI

10.2147/ijn.s58805

More information

Latest update

9/6/2018 1