Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC degrees)
Journal article, 2014
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA. © 2014 Elsevier B.V.
Author
F. Sellrie
Fraunhofer Institute for Biomedical Engineering (IBMT)
C. Lenz
Fraunhofer Institute for Biomedical Engineering (IBMT)
A. Andersson
Fraunhofer Institute for Biomedical Engineering (IBMT)
Marcus Wilhelmsson
Chalmers, Chemical and Biological Engineering, Physical Chemistry
J. A. Schenk
Hybrotec GmbH
Fraunhofer Institute for Biomedical Engineering (IBMT)
Talanta
0039-9140 (ISSN)
Vol. 124 67-70Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories
Physical Chemistry
Analytical Chemistry
Other Basic Medicine
Roots
Basic sciences
DOI
10.1016/j.talanta.2014.02.046