The mammalian AMP-activated protein kinase complex mediates glucose regulation of gene expression in the yeast Saccharomyces cerevisiae
Journal article, 2014

The AMP-activated protein kinase (AMPK) controls energy homeostasis in eukaryotic cells. Here we expressed hetero-trimeric mammalian AMPK complexes in a Saccharomyces cerevisiae mutant lacking all five genes encoding yeast AMPK/SNF1 components. Certain mammalian complexes complemented the growth defect of the yeast mutant on non-fermentable carbon sources. Phosphorylation of the AMPK alpha 1-subunit was glucose-regulated, albeit not by the G1c7-Reg1/2 phosphatase, which performs this function on yeast AMPK/SNFl. AMPK could take over SNF1 function in glucose derepression. While indirectly acting anti-diabetic drugs had no effect on AMPK in yeast, compound 991 stimulated alpha 1-subunit phosphorylation. Our results demonstrate a remarkable functional conservation of AMPK and that glucose regulation of AMPK may not be mediated by regulatory features of a specific phosphatase. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AMP-activated protein kinase

Saccharomyces cerevisiae

Salt stress

Snf1 protein kinase

Mig1 transcriptional repressor

Invertase activity

Active compounds

Author

Ye Tian

University of Gothenburg

Loubna Bendrioua

University of Gothenburg

D. Carmena

Raúl Garcia-Salcedo

University of Gothenburg

Peter Dahl

University of Gothenburg

D. Carling

Stefan Hohmann

University of Gothenburg

FEBS Letters

0014-5793 (ISSN)

Vol. 588 12 2070-2077

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1016/j.febslet.2014.04.039

More information

Created

10/10/2017