Protein-tyrosine phosphorylation interaction network in Bacillus subtilis reveals new substrates, kinase activators and kinase cross-talk
Journal article, 2014

Signal transduction in eukaryotes is generally transmitted through phosphorylation cascades that involve a complex interplay of transmembrane receptors, protein kinases, phosphatases and their targets. Our previous work indicated that bacterial protein-tyrosine kinases and phosphatases may exhibit similar properties, since they act on many different substrates. To capture the complexity of this phosphorylation-based network, we performed a comprehensive interactome study focused on the protein-tyrosine kinases and phosphatases in the model bacterium Bacillus subtilis. The resulting network identified many potential new substrates of kinases and phosphatases, some of which were experimentally validated. Our study highlighted the role of tyrosine and serine/threonine kinases and phosphatases in DNA metabolism, transcriptional control and cell division. This interaction network reveals significant crosstalk among different classes of kinases. We found that tyrosine kinases can bind to several modulators, transmembrane or cytosolic, consistent with a branching of signaling pathways. Most particularly, we found that the division site regulator MinD can form a complex with the tyrosine kinase PtkA and modulate its activity in vitro. In vivo, it acts as a scaffold protein which anchors the kinase at the cell pole. This network highlighted a role of tyrosine phosphorylation in the spatial regulation of the Z-ring during cytokinesis.

bacterial protein kinase

protein phosphorylation

bacterial cell division

bacterial signaling

protein-protein network

Author

Lei Shi

Chalmers, Chemical and Biological Engineering, Life Sciences

Microbiologie de l'Alimentation au Service de la Sante Humaine

N. Pigeonneau

Microbiologie de l'Alimentation au Service de la Sante Humaine

M. Ventroux

Microbiologie de l'Alimentation au Service de la Sante Humaine

Abderahmane Derouiche

Microbiologie de l'Alimentation au Service de la Sante Humaine

Chalmers, Chemical and Biological Engineering, Life Sciences

V. Bidnenko

Chalmers, Chemical and Biological Engineering, Life Sciences

I. Mijakovic

Chalmers, Chemical and Biological Engineering, Life Sciences

Microbiologie de l'Alimentation au Service de la Sante Humaine

M. F. Noirot-Gros

Microbiologie de l'Alimentation au Service de la Sante Humaine

Frontiers in Microbiology

1664302x (eISSN)

Vol. 5 OCT Art. no. 538- 538

Subject Categories

Microbiology

DOI

10.3389/fmicb.2014.00538

More information

Latest update

12/11/2018