Protein-tyrosine phosphorylation in Bacillus subtilis: a 10-year retrospective
Review article, 2015

The discovery of tyrosine-phosphorylated proteins in Bacillus subtilis in the year 2003 was followed by a decade of intensive research activity. Here we provide an overview of the lessons learned in that period. While the number of characterized kinases and phosphatases involved in reversible protein-tyrosine phosphorylation in B. subtilis has remained essentially unchanged, the number of proteins known to be targeted by this post translational modification has increased dramatically. This is mainly due to phosphoproteomics and interactomics studies, which were instrumental in identifying new tyrosine-phosphorylated proteins. Despite their structural similarity, the two B. subtilis protein-tyrosine kinases (BY-kinases), PtkA and PtkB (EpsB), seem to accomplish different functions in the cell. The PtkB is encoded by a large operon involved in exopolysaccharide production, and its main role appears to be the control of this process. The PtkA seems to have a more complex role; it phosphorylates and regulates a large number of proteins involved in the DNA, fatty acid and carbon metabolism and engages in physical interaction with other types of kinases (Ser/Thr kinases), leading to mutual phosphorylation. PtkA also seems to respond to several activator proteins, which direct its activity toward different substrates. In that respect PtkA seems to function as a highly connected signal integration device.

phosphotyrosine-protein phosphatase

regulatory network

protein phosphorylation

substrate specificity

BY-kinase

Author

Ivan Mijakovic

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

J. Deutscher

Institut de Biologie Physico-Chimique

Microbiologie de l'Alimentation au Service de la Sante Humaine

Frontiers in Microbiology

1664302x (eISSN)

Vol. 6 JAN Art. no. 18- 18

Subject Categories

Bioinformatics and Systems Biology

DOI

10.3389/fmicb.2015.00018

More information

Latest update

7/12/2021