Neuronal Networks on Nanocellulose Scaffolds
Journal article, 2015

Proliferation, integration, and neurite extension of PC12 cells, a widely used culture model for cholinergic neurons, were studied in nanocellulose scaffolds biosynthesized by Gluconacetobacter xylinus to allow a three-dimensional (3D) extension of neurites better mimicking neuronal networks in tissue. The interaction with control scaffolds was compared with cationized nanocellulose (trimethyl ammonium betahydroxy propyl [TMAHP] cellulose) to investigate the impact of surface charges on the cell interaction mechanisms. Furthermore, coatings with extracellular matrix proteins (collagen, fibronectin, and laminin) were investigated to determine the importance of integrin-mediated cell attachment. Cell proliferation was evaluated by a cellular proliferation assay, while cell integration and neurite propagation were studied by simultaneous label-free Coherent anti-Stokes Raman Scattering and second harmonic generation microscopy, providing 3D images of PC12 cells and arrangement of nanocellulose fibrils, respectively. Cell attachment and proliferation were enhanced by TMAHP modification, but not by protein coating. Protein coating instead promoted active interaction between the cells and the scaffold, hence lateral cell migration and integration. Irrespective of surface modification, deepest cell integration measured was one to two cell layers, whereas neurites have a capacity to integrate deeper than the cell bodies in the scaffold due to their fine dimensions and amoeba-like migration pattern. Neurites with lengths of >50 μm were observed, successfully connecting individual cells and cell clusters. In conclusion, TMAHP-modified nanocellulose scaffolds promote initial cellular scaffold adhesion, which combined with additional cell-scaffold treatments enables further formation of 3D neuronal networks.


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Chalmers, Biology and Biological Engineering

[Person 2d4c78f2-2cc7-41ef-900b-fdaa4c2d8f87 not found]

Chalmers, Biology and Biological Engineering

[Person 705e3e89-97f7-4c35-91bc-f44e308ad709 not found]

Chalmers, Biology and Biological Engineering

[Person b1b5a44d-fce0-48d7-964d-c914399c05fb not found]

Chalmers, Biology and Biological Engineering

[Person 043d5c25-1319-44f5-b3d8-6d0372cd79fb not found]

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

[Person 195e89ac-2e3d-4685-b55c-4ab1f4f66e7c not found]

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

[Person afee810c-5105-4831-9809-af8ffd383b48 not found]

Chalmers, Biology and Biological Engineering, Chemical Biology

Tissue Engineering - Part C: Methods

1937-3384 (ISSN)

Vol. 21 11 1162-1170

Subject Categories

Biochemistry and Molecular Biology





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