Interactions of Bacterial Lipopolysaccharides with Gold Nanorod Surfaces Investigated by Refractometric Sensing
Journal article, 2015

The interface between nanoparticles and bacterial surfaces is of great interest for applications in nanomedicine and food safety. Here, we demonstrate that interactions between gold nanorods and bacterial surface molecules are governed by the nanoparticle surface coating. Polymer-coated gold nanorod substrates are exposed to lipopolysaccharides extracted from Pseudomonas aeruginosa, Salmonella enterica and Escherichia coli, and attachment is monitored using localized surface plasmon resonance refractometric sensing. The number of lipopolysaccharide molecules attached per nanorod is calculated from the shift in the plasmon maximum, which results from the change in refractive index after analyte binding. Colloidal gold nanorods in water are also incubated with lip opolysaccharides to demonstrate the effect of lipopolysaccharide concentration on plasmon shift, zeta-potential, and association constant. Both gold nanorod surface charge and surface chemistry affect gold nanorod lipopolysaccharide interactions. In general, anionic lipopolysaccharides was found to attach more effectively to cationic gold nanorods than to neutral or anionic gold nanorods. Some variation in lipopolysaccharide attachment is also observed between the three strains studied, demonstrating the potential complexity of bacteria nanoparticle interactions.

localized surface plasmon resonance

lipopolysaccharides

refractometric sensing

gold nanorods

Author

N. S. Abadeer

University of Illinois

Gergö Fülöp

Chalmers, Applied Physics, Bionanophotonics

Si Chen

Chalmers, Applied Physics, Bionanophotonics

Mikael Käll

Chalmers, Applied Physics, Bionanophotonics

C. J. Murphy

University of Illinois

ACS Applied Materials & Interfaces

1944-8244 (ISSN) 1944-8252 (eISSN)

Vol. 7 44 24915-24925

Subject Categories

Other Engineering and Technologies

Nano Technology

DOI

10.1021/acsami.5b08440

More information

Latest update

7/4/2018 6