Effects of storage on mixed-culture biological electrodes
Journal article, 2015

Storage methods are important to preserve the viability and biochemical characteristics of microbial cultures between experiments or during periods when bioreactors are inactive. Most of the research on storage has focused on isolates; however, there is an increasing interest in methods for mixed cultures, which are of relevance in environmental biotechnology. The purpose of this study was to investigate the effect of different storage methods on electrochemically active enrichment cultures. Acetate-oxidizing bioanodes generating a current density of about 5 A m−2 were enriched in a microbial electrolysis cell. The effect of five weeks of storage was evaluated using electrochemical techniques and microbial community analysis. Storage by refrigeration resulted in quicker re-activation than freezing in 10% glycerol, while the bioelectrochemical activity was entirely lost after storage using dehydration. The results showed that the bioelectrochemical activity of bioanodes stored at low temperature could be retained. However, during the re-activation period the bioanodes only recovered 75% of the current density generated before storage and the bacterial communities were different in composition and more diverse after storage than before.

Author

Soroush Saheb Alam

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Frank Persson

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Britt-Marie Wilen

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Malte Hermansson

University of Gothenburg

Oskar Modin

Chalmers, Civil and Environmental Engineering, Water Environment Technology

Scientific Reports

2045-2322 (ISSN)

Vol. 5 no. 18433- 18433

Driving Forces

Sustainable development

Areas of Advance

Building Futures (2010-2018)

Life Science Engineering (2010-2018)

Subject Categories

Microbiology

Environmental Biotechnology

DOI

10.1038/srep18433

More information

Created

10/7/2017