Cellular uptake of PLGA nanoparticles targeted with anti-amyloid and anti-transferrin receptor antibodies for Alzheimer's disease treatment
Journal article, 2016

During the last few decades, relevant efforts have been reported to design nanocarriers for drug transport through the blood brain barrier (BBB). New drugs, such as peptide iA?5, capable to inhibit the aggregates associated with Alzheime?s disease (AD) are being tested but the most frequent drawback is to reach the brain in the desired concentrations due to the low BBB permeability-surface area. Our approach, as a proof of concept to improve drug transport through the BBB, is based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles with surface functionalized with anti-transferrin receptor monoclonal antibody (OX26) and anti-A? (DE2B4) to deliver encapsulated iA?5 into the brain. Porcine brain capillary endothelial cells (PBCECs) were used as a BBB model to evaluate the system efficacy and toxicity. The uptake of immune nanoparticles with a controlled delivery of the peptide iA?5 was substantially increased compared to the nanoparticles (NPs) without monoclonal antibody functionalization.


Immuno nanoparticles

Therapeutic peptides

Blood brain-barrier

Drug delivery systems

Alzheimer's disease


J. A. Loureiro

University of Porto

Bárbara Gomes

University of Porto

Gert Fricker

Heidelberg University

M. A. N. Coelho

University of Porto

Sandra Rocha

Chalmers, Biology and Biological Engineering, Chemical Biology

M. C. Pereira

University of Porto

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN) 1873-4367 (eISSN)

Vol. 145 8-13

Subject Categories

Chemical Engineering



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