Engineering yeast for high-level production of stilbenoid antioxidants
Journal article, 2016

Stilbenoids, including resveratrol and its methylated derivatives, are natural potent antioxidants, produced by some plants in trace amounts as defense compounds. Extraction of stilbenoids from natural sources is costly due to their low abundance and often limited availability of the plant. Here we engineered the yeast Saccharomyces cerevisiae for production of stilbenoids on a simple mineral medium typically used for industrial production. We applied a pull-push-block strain engineering strategy that included overexpression of the resveratrol biosynthesis pathway, optimization of the electron transfer to the cytochrome P450 monooxygenase, increase of the precursors supply, and decrease of the pathway intermediates degradation. Fed-batch fermentation of the final strain resulted in a final titer of 800 mg l(-1) resveratrol, which is by far the highest titer reported to date for production of resveratrol from glucose. We further integrated heterologous methyltransferases into the resveratrol platform strain and hereby demonstrated for the first time de novo biosynthesis of pinostilbene and pterostilbene, which have better stability and uptake in the human body, from glucose.

Author

M. Li

Technical University of Denmark (DTU)

K. Schneider

Technical University of Denmark (DTU)

M. Kristensen

Technical University of Denmark (DTU)

I. Borodina

Technical University of Denmark (DTU)

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Scientific Reports

2045-2322 (ISSN)

Vol. 6 36827- 36827

Subject Categories

Chemical Process Engineering

Chemical Engineering

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1038/srep36827

More information

Latest update

2/28/2018