DCEO Biotechnology: Tools to Design, Construct, Evaluate, and Optimize the Metabolic Pathway for Biosynthesis of Chemicals
Review article, 2018

Chemical synthesis is a well established route for producing many chemicals on a large scale, but some drawbacks still exist in this process, such as unstable intermediates, multistep reactions, complex process control, etc. Biobased production provides an attractive alternative to these challenges, but how to make cells into efficient factories is challenging. As a key enabling technology to develop efficient cell factories, design-construction-evaluation-optimization (DCEO) biotechnology, which incorporates the concepts and techniques of pathway design, pathway construction, pathway evaluation, and pathway optimization at the systems level, offers a conceptual and technological framework to exploit potential pathways, modify existing pathways and create new pathways for the optimal production of desired chemicals. Here, we summarize recent progress of DCEO biotechnology and examples of its application, and provide insights as to when, what and how different strategies should be taken. In addition, we highlight future perspectives of DCEO biotechnology for the successful establishment of biorefineries.


Xiulai Chen

Jiangnan University

Cong Gao

Jiangnan University

Liang Guo

Jiangnan University

Guipeng Hu

Jiangnan University

Qiuling Luo

Jiangnan University

Jia Liu

Jiangnan University

Jens B Nielsen

Technical University of Denmark (DTU)

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jian Chen

Jiangnan University

Liming Liu

Jiangnan University

Chalmers, Biology and Biological Engineering

Chemical Reviews

0009-2665 (ISSN) 1520-6890 (eISSN)

Vol. 118 1 4-72

Subject Categories

Production Engineering, Human Work Science and Ergonomics

Other Mechanical Engineering

Bioprocess Technology



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