Synergistic effects of copper sites on apparent stability of multicopper oxidase, Fet3p
Journal article, 2018

Saccharomyces cerevisiae Fet3p is a multicopper oxidase that contains three cupredoxin-like domains and four copper ions located in three distinct metal sites (T1 in domain 3; T2 and the binuclear T3 at the interface between domains 1 and 3). To probe the role of the copper sites in Fet3p thermodynamic stability, we performed urea-induced unfolding experiments with holo-, apoand three partially-metallated (T1, T2 and T1/T2 sites depleted of copper) forms of Fet3p. Using a combination of spectroscopic probes (circular dichroism, fluorescence intensity and maximum, 8-anilinonaphthalene-1-sulfonic acid (ANS) emission, oxidase activity and blue color), we reveal that all forms of Fet3p unfold in a four-state reaction with two partially-folded intermediates. Using phase diagrams, it emerged that Fet3p with all copper sites filled had a significantly higher stability as compared to the combined contributions of the individual copper sites. Hence, there is long-range inter-domain communication between distal copper sites that contribute to overall Fet3p stability.

Phase diagram method

Multicopper oxidases MCO

Multidomain protein stability

Cupredoxin-like domain



Erik Sedlák

Pavol Jozef Safarik University

Gabriel Žoldák

Pavol Jozef Safarik University

Pernilla Wittung Stafshede

Chalmers, Biology and Biological Engineering, Chemical Biology

International Journal of Molecular Sciences

1422-0067 (ISSN)

Vol. 19 1 269

Subject Categories

Biochemistry and Molecular Biology

Other Basic Medicine

Structural Biology



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