Pernilla Wittung Stafshede

Full Professor at Chemical Biology

Pernilla Wittung-Stafshede is since 2015 professor (division head 2015-2018) at the Chemical Biology division in the Biology and Biological Engineering department. She leads a research group that focuses on metalloprotein mechanisms (e.g., in cancer) and protein misfolding (e.g. in Parkinson's disease). Pernilla has spent 12 years as a professor in the United States (at Tulane and Rice universities) followed by 7 years at Umeå University before she joined Chalmers faculty. She has published over 220 peer-reviewed papers (2019) and many popular texts. Since january 2019, Pernilla is the Head of Genie, Gender Initiative for Excellence, which is a huge 10-year initiative financed by the Foundation in order to improve gender equality among Chalmers faculty.Wittung-Stafshede Research GroupGenieGenie (Insidan)

Source: chalmers.se

Contact Details ORCID

Pernilla Wittung Stafshede from chalmers.se

Publications (93)
Projects (9)

Showing 93 publications

2020

Evaluation of copper chaperone ATOX1 as prognostic biomarker in breast cancer

Stephanie Blockhuys, Donita C. Brady, Pernilla Wittung Stafshede

Breast Cancer. Vol. in press

Journal article

2020

Single-cell tracking demonstrates copper chaperone Atox1 to be required for breast cancer cell migration

Stephanie Blockhuys, Xiaolu Zhang, Pernilla Wittung Stafshede

Proceedings of the National Academy of Sciences of the United States of America. Vol. 117 (4), p. 2014-2019

Journal article

2020

Correlation between Cellular Uptake and Cytotoxicity of Fragmented α-Synuclein Amyloid Fibrils Suggests Intracellular Basis for Toxicity

Xiaolu Zhang, Emelie Vilhelmsson Wesén, Ranjeet Kumar et al

ACS Chemical Neuroscience. Vol. 11 (3), p. 233-241

Journal article

2020

Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein

Jonas Hannestad, Sandra Rocha, Björn Agnarsson et al

Proceedings of the National Academy of Sciences of the United States of America. Vol. 117 (25), p. 14178-14186

Journal article

2020

Differential effects of Cu2+ and Fe3+ ions on in vitro amyloid formation of biologically-relevant α-synuclein variants

Emma Lorentzon, Ranjeet Kumar, Istvan Horvath et al

Biometals. Vol. In Press

Journal article

2020

A gut bacterial amyloid promotes α-synuclein aggregation and motor impairment in mice

Timothy R. Sampson, Collin Challis, Neha Jain et al

eLife. Vol. 9

Journal article

2020

Role of Zero-Order Loop in Protein Unfolding Case Study with Apoazurin

Dirar M. Homouz, Fabio Zegarra, Pernilla Wittung Stafshede et al

Biophysical Journal. Vol. 118 (3, Supplement 1), p. 197A-197A

Paper in proceedings

2020

Redox-Dependent Copper Ion Modulation of Amyloid-β (1-42) Aggregation In Vitro

Nima Sasanian, David Bernson, Istvan Horvath et al

Biomolecules. Vol. 10 (6), p. 924-

Journal article

2020

Mirror-Image 5S Ribonucleoprotein Complexes

Jun-Jie Ling, Chuyao Fan, Hong Qin et al

Angewandte Chemie - International Edition. Vol. 59 (9), p. 3724-3731

Journal article

2019

Copper relay path through the N-terminus of Wilson disease protein, ATP7B

Kumaravel Ponnandai Schanmugavel, Pernilla Wittung Stafshede

Metallomics (9), p. 1472-1480

Journal article

2019

Membrane-protein-hydration interaction of alpha-synuclein with anionic vesicles in solution probed via angle-resolved second harmonic scattering

Jan Dedic, Sandra Rocha, Pernilla Wittung Stafshede et al

Conference contribution

2019

Alpha-Synuclein Binds to DNA and Modulates its Physical Properties

Kai Jiang, Sandra Rocha, Alvina Westling et al

Biophysical Journal

Paper in proceedings

2019

My journey in academia: Things not on the CV

Pernilla Wittung Stafshede

Pure and Applied Chemistry

Journal article

2019

Crowding-Induced Elongated Conformation of Urea-Unfolded Apoazurin: Investigating the Role of Crowder Shape in Silico

Fabio C. Zegarra, Dirar Homouz, Andrei G. Gasic et al

Journal of Physical Chemistry B. Vol. 123 (17), p. 3607-3617

Journal article

2019

Synaptic vesicle mimics affect the aggregation of wild-type and A53T α-synuclein variants differently albeit similar membrane affinity

Sandra Rocha, Ranjeet Kumar, Istvan Horvath et al

Protein engineering, design & selection : PEDS. Vol. 32 (2), p. 59-66

Journal article

2019

Interaction between Copper Chaperone Atox1 and Parkinson's Disease Protein α-Synuclein Includes Metal-Binding Sites and Occurs in Living Cells

Istvan Horvath, Stephanie Blockhuys, Darius Šulskis et al

ACS Chemical Neuroscience. Vol. 10 (11), p. 4659-4668

Journal article

2019

Wilson disease missense mutations in ATP7B affect metal-binding domain structural dynamics

Kumaravel Ponnandai Schanmugavel, Ranjeet Kumar, Yaozong Li et al

Biometals. Vol. In print

Journal article

2019

Membrane-Protein-Hydration Interaction of α-Synuclein with Anionic Vesicles Probed via Angle-Resolved Second-Harmonic Scattering

Jan Dedic, Sandra Rocha, Halil I. Okur et al

Journal of Physical Chemistry B. Vol. 123 (5), p. 1044-1049

Journal article

2018

Copper Chaperone Atox1 Interacts with Cell Cycle Proteins

Maria Matson Dzebo, Stephanie Blockhuys, Sebastian Valenzuela et al

Computational and Structural Biotechnology Journal. Vol. 16, p. 443-449

Journal article

2018

Folding of copper proteins: Role of the metal?

Candan Ariöz, Pernilla Wittung Stafshede

Quarterly Reviews of Biophysics. Vol. 51

Review article

2018

Abundant fish protein inhibits α-synuclein amyloid formation

Tony Werner, Ranjeet Kumar, Istvan Horvath et al

Scientific Reports. Vol. 8 (1)

Journal article

2018

Copper chaperone blocks amyloid formation via ternary complex

Istvan Horvath, Tony Werner, Ranjeet Kumar et al

Quarterly Reviews of Biophysics. Vol. 51

Review article

2018

Fucosylated Molecules Competitively Interfere with Cholera Toxin Binding to Host Cells

Amberlyn M. Wands, Jakob Cervin, He Huang et al

ACS Infectious Diseases. Vol. 4 (5), p. 758-770

Journal article

2018

The processes of α-synuclein amyloid protein complexes involved in the pathogenesis of Parkinson’s disease

Juris Kiskis, Istvan Horvath, Pernilla Wittung Stafshede et al

Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova. Vol. 118 (8), p. 75-81

Journal article

2018

Alpha-Synuclein Modulates the Physical Properties of DNA

Kai Jiang, Sandra Rocha, Alvina Westling et al

Chemistry - A European Journal. Vol. 24 (58), p. 15685-15690

Journal article

2018

Geometrical Description of Protein Structural Motifs

John J. Kozak, Harry B. Gray, Pernilla Wittung Stafshede

Journal of Physical Chemistry B. Vol. 122 (49), p. 11289-11294

Journal article

2018

In vitro analysis of α-synuclein amyloid formation and cross-reactivity

Istvan Horvath, Sandra Rocha, Pernilla Wittung Stafshede

Methods in Molecular Biology, p. 73-83

Book chapter

2018

Synergistic effects of copper sites on apparent stability of multicopper oxidase, Fet3p

Erik Sedlák, Gabriel Žoldák, Pernilla Wittung Stafshede

International Journal of Molecular Sciences. Vol. 19 (1)

Journal article

2018

3D-Models of Insulin-Producing β-Cells: from Primary Islet Cells to Stem Cell-Derived Islets

Diana Ribeiro, Alexander J. Kvist, Pernilla Wittung Stafshede et al

Stem Cell Reviews and Reports. Vol. 14 (2), p. 177-188

Journal article

2018

A Luminal Loop of Wilson Disease Protein Binds Copper and Is Required for Protein Activity

Birgit Köhn, Kumaravel Ponnandai Schanmugavel, Min Wu et al

Biophysical Journal. Vol. 115 (6), p. 1007-1018

Journal article

2018

Copper distribution in breast cancer cells detected by time-of-flight secondary ion mass spectrometry with delayed extraction methodology

Stephanie Blockhuys, Per Malmberg, Pernilla Wittung Stafshede

Biointerphases. Vol. 13 (6)

Journal article

2017

The six metal binding domains in human copper transporter, ATP7B: molecular biophysics and disease-causing mutations

Candan Ariöz, Y. Z. Li, Pernilla Wittung Stafshede

Biometals. Vol. 30 (6), p. 823-840

Journal article

2017

Effects of small-molecule amyloid modulators on a Drosophila model of Parkinson's disease

M. Pokrzywa, K. Pawelek, W. E. Kucia et al

PLoS ONE. Vol. 12 (9), p. Aet no e0184117-

Journal article

2017

Copper chaperone Atox1 plays role in breast cancer cell migration

Stephanie Blockhuys, Pernilla Wittung Stafshede

Biochemical and Biophysical Research Communications. Vol. 483 (1), p. 301-304

Journal article

2017

Roles of copper-binding proteins in breast cancer

Stephanie Blockhuys, Pernilla Wittung Stafshede

International Journal of Molecular Sciences. Vol. 18 (4), p. Article no 871-

Journal article

2017

Defining the human copper proteome and analysis of its expression variation in cancers.

Stephanie Blockhuys, Emanuele Celauro, C. Hildesjo et al

Metallomics. Vol. 9 (2), p. 112-123

Journal article

2017

Extracellular vesicles from human pancreatic islets suppress human islet amyloid polypeptide amyloid formation

Diana Ribeiro, Istvan Horvath, N. Heath et al

Proceedings of the National Academy of Sciences of the United States of America. Vol. 114 (42), p. 11127-11132

Journal article

2017

Cross-Reactivity of Alpha-Synuclein with Other Cellular Components Can Dramatically Modulate Amyloid Formation

Pernilla Wittung Stafshede

Biophysical Journal. Vol. 112 (3), p. 365A-365A

Magazine article

2017

Probing functional roles of Wilson disease protein (ATP7B) copper-binding domains in yeast

Kumaravel Ponnandai Schanmugavel, Dina Petranovic Nielsen, Pernilla Wittung Stafshede

Metallomics. Vol. 9 (7), p. 981-988

Journal article

2017

Disease-causing point-mutations in metal-binding domains of Wilson disease protein decrease stability and increase structural dynamics.

Ranjeet Kumar, Candan Ariöz, Yaozong Li et al

Biometals. Vol. 30 (1), p. 27-35

Journal article

2017

A stretched conformation of DNA with a biological role?

Niklas Bosaeus, Anna Reymer, Tamas Beke-Somfai et al

Quarterly Reviews of Biophysics. Vol. 50, p. UNSP e11-

Journal article

2017

Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy

Stephanie Blockhuys, Nisha Rani Agarwal, C. Hildesjo et al

Journal of Biomedical Optics. Vol. 22 (10)

Journal article

2017

Copper chaperone ATOX1 regulates pluripotency factor OCT4 in preimplantation mouse embryos

Emanuele Celauro, Amisa Mukaj, Juan Carlos Fierro González et al

Biochemical and Biophysical Research Communications. Vol. 491 (1), p. 147-153

Journal article

2017

Unraveling amyloid formation paths of Parkinson's disease protein alpha-synuclein triggered by anionic vesicles

Juris Kiskis, Istvan Horvath, Pernilla Wittung Stafshede et al

Quarterly Reviews of Biophysics. Vol. 50, p. 1-9

Journal article

2016

Gut Microbiota Regulate Motor Deficits and Neuroinflammation in a Model of Parkinson's Disease.

Timothy R Sampson, Justine W Debelius, Taren Thron et al

Cell. Vol. 167 (6), p. 1469-1480.e12

Journal article

2016

Extended functional repertoire for human copper chaperones

Maria Matson Dzebo, Candan Ariöz, Pernilla Wittung Stafshede

Biomolecular Concepts. Vol. 7 (1), p. 29-39

Journal article

2016

A Copper Story: From Protein Folding and Metal Transport to Cancer

Pernilla Wittung Stafshede

Israel Journal of Chemistry. Vol. 56 (9-10), p. 671-681

Journal article

2016

Akademin is Sverige är ej så jämställd som du tror.

Pernilla Wittung Stafshede

Curie Vetenskapsrådets webb tidning

Magazine article

2016

Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA

James A D Good, Christopher Andersson, Sabine Hansen et al

Cell Chemical Biology. Vol. 23 (3), p. 404-414

Journal article

2016

Protein Interactions that Enable Safe and Efficient Copper Ion Transport in the Human Cytoplasm

Pernilla Wittung Stafshede

Biophysical Journal. Vol. 110 (3), p. 179A-179A

Journal article

2016

Cross-talk between amyloidogenic proteins in type-2 diabetes and Parkinson's disease

Istvan Horvath, Pernilla Wittung Stafshede

Proceedings of the National Academy of Sciences of the United States of America. Vol. 113 (44), p. 12473-12477

Journal article

2016

The C-Terminus of Human Copper Importer Ctr1 Acts as a Binding Site and Transfers Copper to Atox1

D. Kahra, M. Kovermann, Pernilla Wittung Stafshede

Biophysical Journal. Vol. 110 (1), p. 95-102

Journal article

2016

Is the gender gap solved in liberal Sweden?

Pernilla Wittung Stafshede

stemwomen.net

Magazine article

2016

gästbloggare

Pernilla Wittung Stafshede

Curie Vetenskapsrådets webb tidning

Magazine article

2016

Copper binding triggers compaction in N-terminal tail of human copper pump ATP7B

T. Mondol, J. Åden, Pernilla Wittung Stafshede

Biochemical and Biophysical Research Communications. Vol. 470 (3), p. 663-669

Journal article

2015

Enthalpy-entropy compensation at play in human copper ion transfer.

Moritz S Niemiec, Artur P G Dingeldein, Pernilla Wittung Stafshede

Scientific Reports. Vol. 5, p. 10518-

Journal article

2015

The bacterial curli system possesses a potent and selective inhibitor of amyloid formation.

Margery L Evans, E. Chorell, Jonathan D Taylor et al

Molecular Cell. Vol. 57 (3), p. 445-55

Journal article

2015

Insulin-degrading enzyme prevents α-synuclein fibril formation in a nonproteolytical manner.

Sandeep K Sharma, E. Chorell, Pär Steneberg et al

Scientific Reports. Vol. 5, p. 12531-

Journal article

2015

Human Copper Chaperone Atox1 Translocates to the Nucleus but does not Bind DNA In Vitro.

Dana Kahra, Tanumoy Mondol, Moritz S Niemiec et al

Protein and peptide letters. Vol. 22 (6), p. 532-8

Journal article

2015

Human cytoplasmic copper chaperones Atox1 and CCS exchange copper ions in vitro.

Svenja Petzoldt, Dana Kahra, Michael Kovermann et al

Biometals. Vol. 28 (3), p. 577-85

Journal article

2015

Unresolved questions in human copper pump mechanisms.

Pernilla Wittung Stafshede

Quarterly Reviews of Biophysics. Vol. 48 (4), p. 471-8

Journal article

2015

Bacterial Chaperones CsgE and CsgC Differentially Modulate Human α-Synuclein Amyloid Formation via Transient Contacts.

E. Chorell, Emma Andersson, Margery L Evans et al

PLoS ONE. Vol. 10 (10), p. e0140194-

Journal article

2015

Synthesis of Multiring Fused 2-Pyridones via a Nitrene Insertion Reaction: Fluorescent Modulators of α-Synuclein Amyloid Formation

P. Singh, E. Chorell, K.S. Krishnan et al

Organic Letters. Vol. 17 (24), p. 6194-6197

Journal article

2015

Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation?

Helena Öhrvik, Pernilla Wittung Stafshede

International Journal of Molecular Sciences. Vol. 16 (8), p. 16728-39

Journal article

2015

Single injection of small-molecule amyloid accelerator results in cell death of nigral dopamine neurons in mice

M. Chermenina, E. Chorell, M. Pokrzywa et al

Parkinsons Disease. Vol. 1

Journal article

2015

Insulin-degrading enzyme is activated by the C-terminus of α-synuclein.

Sandeep K Sharma, E. Chorell, Pernilla Wittung Stafshede

Biochemical and Biophysical Research Communications. Vol. 466 (2), p. 192-5

Journal article

2015

Direct Correlation Between Ligand-Induced α-Synuclein Oligomers and Amyloid-like Fibril Growth.

Martin Nors Perdersen, Vito Foderà, Istvan Horvath et al

Scientific Reports. Vol. 5, p. 10422-

Journal article

2011

Cisplatin binds human copper chaperone Atox1 and promotes unfolding in vitro.

Maria E Palm, Christoph F Weise, Christina Lundin et al

Proceedings of the National Academy of Sciences of the United States of America. Vol. 108 (17), p. 6951-6

Journal article

1999

The L2 Loop Peptide of recA Stiffens and restricts Base Motions of Single-stranded DNA Similar to Intact Protein

T. Selmane, Pernilla Wittung Stafshede, F. Maraboeuf et al

FEBS Letters. Vol. 446 (1), p. 30-34

Journal article

1999

Peptide nucleic acids (PNA) derived from N-(N-methylaminoethyl)glycine. Synthesis, hybridization and structural properties

G. Haaima, H. Rasmussen, G. Schmidt et al

New Journal of Chemistry. Vol. 23 (8), p. 833-840

Journal article

1999

Peptide nucleic acids

Bengt Nordén, Pernilla Wittung Stafshede

The Encyclopedia of Molecular Biology (ed. Thomas E.Creighton), p. 1801-1808

Book chapter

1998

Base Orientation of Second DNA in RecA-DNA Filaments. Analysis by combination of linear dichroism and small angle neutron scattering in flow-oriented solution

Bengt Nordén, Pernilla Wittung Stafshede, C. Ellouze et al

Journal of Biological Chemistry. Vol. 273 (25), p. 15682-15686

Journal article

1997

Recognition of Double-Stranded DNA by Peptide Nucleic Acids

Pernilla Wittung, P. Nielsen, Bengt Nordén

Nucleosides and Nucleotides. Vol. 16 (5-6), p. 599-602

Journal article

1997

Observation of a PNA-PNA-PNA triplex

Pernilla Wittung, P. Nielsen, Bengt Nordén

Journal of the American Chemical Society. Vol. 119 (13), p. 3189-3190

Journal article

1997

Thermochemical and kinetic evidence for nucleotide-sequence-dependent RecA-DNA interactions

Pernilla Wittung, C. Ellouze, F. Maraboeuf et al

European Journal of Biochemistry. Vol. 245 (3), p. 715-719

Journal article

1997

Extended DNA-Recognition Repertoire of Peptide Nucleic Acid (PNA): PNA-dsDNA triplex formed with cytosine-rich homopyrimidine PNA

Pernilla Wittung, P. Nielsen, Bengt Nordén

Biochemistry. Vol. 36 (26), p. 7973-7979

Journal article

1997

Peptide nucleic acids with a conformationally constrained chiral cyclohexyl-derived backbone

P. Lagriffoule, Pernilla Wittung, M. Eriksson et al

Chemistry - A European Journal. Vol. 3 (6), p. 912-919

Journal article

1996

Ionic effects on the stability and conformation of peptide nucleic acid complexes

S. Tomac, M. Sarkar, Tommi Ratilainen et al

Journal of the American Chemical Society. Vol. 118 (24), p. 5544-5552

Journal article

1996

Second-site RecA - DNA interactions: Lack of identical recognition

Pernilla Wittung, L. R. Bazemore, M. Takahashi et al

Biochemistry. Vol. 35 (48), p. 15349-15355

Journal article

1996

Intelligent Nucleic Acid Interactions with Peptide Nucleic Acid (PNA) and Recombination Enzyme RecA

Pernilla Wittung

Doctoral thesis

1996

Direct observation of strand invasion by peptide nucleic acid (PNA) into double-stranded DNA

Pernilla Wittung, P. Nielsen, Bengt Nordén

Journal of the American Chemical Society. Vol. 118 (30), p. 7049-7054

Journal article

1995

Functional interactions of nucleic acids : in simple DNA analogs and recombinase intermediates

Pernilla Wittung

Licentiate thesis

1995

Secondary Structure of RecA in Solution - The Effects of Cofactor, DNA and Ionic Conditions

Pernilla Wittung, Bengt Nordén, M. Takahashi

European Journal of Biochemistry. Vol. 228 (1), p. 149-154

Journal article

1995

Fluorescence-detected interactions of oligonucleotides in RecA complexes

Pernilla Wittung, M. Funk, B. Jernstrom et al

FEBS Letters. Vol. 368 (1), p. 64-68

Journal article

1995

Induced Chirality in PNA-PNA Duplexes

Pernilla Wittung, M. Eriksson, Reidar Lyng et al

Journal of the American Chemical Society. Vol. 117 (41), p. 10167-10173

Journal article

1995

Evidence for Elongation of the Helical Pitch of the RecA Filament Upon ATP and ADP Binding Using Small-Angle Neutron Scattering

C. Ellouze, M. Takahashi, Pernilla Wittung et al

European Journal of Biochemistry. Vol. 233 (2), p. 579-583

Journal article

1995

PNA-Peptide Chimerae

T. Koch, M. Naesby, Pernilla Wittung et al

Tetrahedron Letters. Vol. 36 (38), p. 6933-6936

Journal article

1995

Phospholipid membrane permeability of peptide nucleic acid

Pernilla Wittung, Johan Kajanus, K. Edwards et al

FEBS Letters. Vol. 365 (1), p. 27-29

Journal article

1994

INTERACTIONS BETWEEN DNA-MOLECULES BOUND TO RECA FILAMENT - EFFECTS OF BASE COMPLEMENTARITY

Pernilla Wittung, Bengt Nordén, S. K. Kim et al

Journal of Biological Chemistry. Vol. 269 (8), p. 5799-5803

Journal article

1994

INTERACTIONS OF DNA-BINDING LIGANDS WITH PNA DNA HYBRIDS

Pernilla Wittung, S. K. Kim, O. Buchardt et al

Nucleic Acids Research. Vol. 22 (24), p. 5371-5377

Journal article

1994

DNA-LIKE DOUBLE HELIX FORMED BY PEPTIDE NUCLEIC-ACID

Pernilla Wittung, P. E. Nielsen, O. Buchardt et al

Nature. Vol. 368 (6471), p. 561-563

Journal article

1994

STRUCTURE-ACTIVITY STUDIES OF THE BINDING OF MODIFIED PEPTIDE NUCLEIC-ACIDS (PNAS) TO DNA

B. Hyrup, M. Egholm, P. E. Nielsen et al

Journal of the American Chemical Society. Vol. 116 (18), p. 7964-7970

Journal article

1994

SPECTROSCOPIC OBSERVATION OF RENATURATION BETWEEN POLYNUCLEOTIDES INTERACTING WITH RECA IN THE PRESENCE OF ATP HYDROLYSIS

Pernilla Wittung, Bengt Nordén, M. Takahashi

European Journal of Biochemistry. Vol. 224 (1), p. 39-45

Journal article

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