Recognition of Double-Stranded DNA by Peptide Nucleic Acids
Journal article, 1997

Peptide nucleic acid (PNA) is an oligonucleotide mimic in which the backbone of DNA has been replaced by a pseudopeptide. We here show that there are distinct variations as to how PNA oligomers interact with double-stranded DNA depending on choice of nucleobases. Thymine-rich homopyrimidine PNA oligomers recognize double-stranded polynucleotides by forming PNA2-DNA triplexes with the DNA purine strand. By contrast, cytosine-rich homopyrimidine PNAs add to double-stranded polynucleotides as Hoogsteen strands, forming PNA-DNA2 triplexes, while homopurine, or alternating thymine-guanine, PNA oligomers invade DNA to form PNA-DNA duplexes.


Pernilla Wittung

Department of Physical Chemistry

P. Nielsen

Department of Physical Chemistry

Bengt Nordén

Chalmers, Department of Chemistry and Bioscience

Nucleosides and Nucleotides

0732-8311 (ISSN) 1532-2335 (eISSN)

Vol. 16 5-6 599-602

Areas of Advance

Nanoscience and Nanotechnology (SO 2010-2017, EI 2018-)


Life Science Engineering (2010-2018)

Materials Science

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Physical Chemistry


Basic sciences

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