Recognition of Double-Stranded DNA by Peptide Nucleic Acids
Artikel i vetenskaplig tidskrift, 1997

Peptide nucleic acid (PNA) is an oligonucleotide mimic in which the backbone of DNA has been replaced by a pseudopeptide. We here show that there are distinct variations as to how PNA oligomers interact with double-stranded DNA depending on choice of nucleobases. Thymine-rich homopyrimidine PNA oligomers recognize double-stranded polynucleotides by forming PNA2-DNA triplexes with the DNA purine strand. By contrast, cytosine-rich homopyrimidine PNAs add to double-stranded polynucleotides as Hoogsteen strands, forming PNA-DNA2 triplexes, while homopurine, or alternating thymine-guanine, PNA oligomers invade DNA to form PNA-DNA duplexes.

Författare

Pernilla Wittung Stafshede

Institutionen för fysikalisk kemi

P. Nielsen

Institutionen för fysikalisk kemi

Bengt Nordén

Chalmers, Institutionen för kemi och biovetenskap

Nucleosides and Nucleotides

0732-8311 (ISSN) 1532-2335 (eISSN)

Vol. 16 599-602

Styrkeområden

Nanovetenskap och nanoteknik

Energi

Livsvetenskaper och teknik

Materialvetenskap

Ämneskategorier

Fysikalisk kemi

Fundament

Grundläggande vetenskaper