Alpha-Synuclein Modulates the Physical Properties of DNA
Journal article, 2018

Published by Wiley-VCH Verlag GmbH & Co. KGaA. Fundamental research on Parkinson's disease (PD) most often focuses on the ability of α-synuclein (aS) to form oligomers and amyloids, and how such species promote brain cell death. However, there are indications that aS also plays a gene-regulatory role in the cell nucleus. Here, the interaction between monomeric aS and DNA in vitro has been investigated with single-molecule techniques. Using a nanofluidic channel system, it was discovered that aS binds to DNA and by studying the DNA–protein complexes at different confinements we determined that aS binding increases the persistence length of DNA from 70 to 90 nm at high coverage. By atomic force microscopy it was revealed that at low protein-to-DNA ratio, the aS binding occurs as small protein clusters scattered along the DNA; at high protein-to-DNA ratio, the DNA is fully covered by protein. As DNA-aS interactions may play roles in PD, it is of importance to characterize biophysical properties of such complexes in detail.

proteins

nanofluidics

biochemistry

synuclein

DNA

Author

Kai Jiang

Chalmers, Biology and Biological Engineering, Chemical Biology

Sandra Rocha

Chalmers, Biology and Biological Engineering, Chemical Biology

Alvina Westling

Student at Chalmers

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Kevin D. Dorfman

University of Minnesota

Pernilla Wittung Stafshede

Chalmers, Biology and Biological Engineering, Chemical Biology

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 24 58 15685-15690

Subject Categories

Biochemistry and Molecular Biology

Biophysics

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1002/chem.201803933

PubMed

30102440

More information

Latest update

7/2/2019 1