Phospholipid membrane permeability of peptide nucleic acid
Journal article, 1995
Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone, The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.
oligonucleotide analogs
pna
thymine
microscopy
dna
antisense
vesicles
liposome
fluorescence
peptide nucleic acid
recognition
cryogenic transmission electron
Author
Pernilla Wittung
Department of Physical Chemistry
Johan Kajanus
Department of Organic Chemistry
K. Edwards
P. Nielsen
Bengt Nordén
Department of Physical Chemistry
B. G. Malmstrom
FEBS Letters
0014-5793 (ISSN) 18733468 (eISSN)
Vol. 365 1 27-29Subject Categories
Biochemistry and Molecular Biology
DOI
10.1016/0014-5793(95)00409-3