Phospholipid membrane permeability of peptide nucleic acid
Artikel i vetenskaplig tidskrift, 1995
Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone, The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.
oligonucleotide analogs
pna
thymine
microscopy
dna
antisense
vesicles
liposome
fluorescence
peptide nucleic acid
recognition
cryogenic transmission electron
Författare
Pernilla Wittung
Institutionen för fysikalisk kemi
Johan Kajanus
Institutionen för organisk kemi
K. Edwards
P. Nielsen
Bengt Nordén
Institutionen för fysikalisk kemi
B. G. Malmstrom
FEBS Letters
0014-5793 (ISSN) 18733468 (eISSN)
Vol. 365 1 27-29Ämneskategorier
Biokemi och molekylärbiologi
DOI
10.1016/0014-5793(95)00409-3