Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
Journal article, 2023

The assembly of α-synuclein into cross-β structured amyloid fibers results in Lewy body deposits and neuronal degeneration in Parkinson’s disease patients. As the cell environment is highly crowded, interactions between the formed amyloid fibers and a range of biomolecules can occur in cells. Although amyloid fibers are considered chemically inert species, recent in vitro work using model substrates has shown α-synuclein amyloids, but not monomers, to catalyze the hydrolysis of ester and phosphoester bonds. To search for putative catalytic activity of α-synuclein amyloids on biologically relevant metabolites, we here incubated α-synuclein amyloids with neuronal SH-SY5Y cell lysates devoid of proteins. LC-MS-based metabolomic (principal component and univariate) analysis unraveled distinct changes in several metabolite levels upon amyloid (but not monomer) incubation. Of 63 metabolites identified, the amounts of four increased (3-hydroxycapric acid, 2-pyrocatechuic acid, adenosine, and NAD), and the amounts of seventeen decreased (including aromatic and apolar amino acids, metabolites in the TCA cycle, keto acids) in the presence of α-synuclein amyloids. Many of these metabolite changes match what has been reported previously in Parkinson’s disease patients and animal–model metabolomics studies. Chemical reactivity of α-synuclein amyloids may be a new gain-of-function that alters the metabolite composition in cells and, thereby, modulates disease progression.

Parkinson’s disease






Istvan Horvath

Chalmers, Life Sciences, Chemical Biology

Khadra A. Mohamed

Chalmers, Life Sciences, Chemical Biology

Ranjeet Kumar

Chalmers, Life Sciences, Chemical Biology

Pernilla Wittung Stafshede

Chalmers, Life Sciences, Chemical Biology

International Journal of Molecular Sciences

16616596 (ISSN) 14220067 (eISSN)

Vol. 24 16 12849

Subject Categories

Biochemistry and Molecular Biology







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9/8/2023 1