Long-term dynamics of the bacterial community in a Swedish full-scale wastewater treatment plant
Journal article, 2019

The operational efficiency of activated sludge wastewater treatment plants depends to a large extent on the microbial community structure of the activated sludge. The aims of this paper are to describe the composition of the bacterial community in a Swedish full-scale activated sludge wastewater treatment plant, to describe the dynamics of the community and to elucidate possible causes for bacterial community composition changes. The bacterial community composition in the activated sludge was described using 16S rRNA gene libraries and monitored for 15 months by a terminal restriction fragment (T-RF) length polymorphism (T-RFLP) analysis of the 16S rRNA gene. Despite variable environmental conditions, a large fraction of the observed T-RFs were present at all times, making up at least 50% in all samples, possibly representing a relatively stable core fraction of the bacterial community. However, the proportions of the different T-RFs in this fraction as well as the T-RFs in the more variable fraction showed a significant variation over time and temperature. The difference in community composition between summer and winter coincided with observed differences in floc structure. These observations suggest a relationship between floc properties and bacterial community composition, although additional experiments are required to determine causality.

wastewater treatment

activated sludge

bacterial population dynamics

16S rRNA gene sequence analysis

terminal restriction fragment length polymorphism analysis

Author

Johan Fredriksson

Chalmers, Architecture and Civil Engineering, Water Environment Technology

Malte Hermansson

University of Gothenburg

Britt-Marie Wilen

Chalmers, Architecture and Civil Engineering, Water Environment Technology

Environmental Technology (United Kingdom)

0959-3330 (ISSN)

Vol. 40 7 912-928

Subject Categories

Civil Engineering

Water Engineering

DOI

10.1080/09593330.2017.1411396

More information

Latest update

10/30/2019