The evidence for open and closed exocytosis as the primary release mechanism
Journal article, 2016

Exocytosis is the fundamental process by which cells communicate with each other. The events that lead up to the fusion of a vesicle loaded with chemical messenger with the cell membrane were the subject of a Nobel Prize in 2013. However, the processes occurring after the initial formation of a fusion pore are very much still in debate. The release of chemical messenger has traditionally been thought to occur through full distention of the vesicle membrane, hence assuming exocytosis to be all or none. In contrast to the all or none hypothesis, here we discuss the evidence that during exocytosis the vesicle-membrane pore opens to release only a portion of the transmitter content during exocytosis and then close again. This open and closed exocytosis is distinct from kiss- and-run exocytosis, in that it appears to be the main content released during regular exocytosis. The evidence for this partial release via open and closed exocytosis is presented considering primarily the quantitative evidence obtained with amperometry.

kiss-and-run

adrenal chromaffin cells

quantal size

fusion pore

frog neuromuscular-junction

dense-core vesicles

regulated exocytosis

peritoneal mast-cells

transmitter release

single synaptic vesicles

Author

Lin Ren

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Lisa Mellander

University of Gothenburg

Jacqueline Keighron

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Ann-Sofie Cans

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Michael Kurczy

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

I. Svir

Sorbonne University

A. Oleinick

Sorbonne University

C. Amatore

Sorbonne University

Andrew Ewing

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Quarterly Reviews of Biophysics

0033-5835 (ISSN) 1469-8994 (eISSN)

Vol. 49

Subject Categories

Condensed Matter Physics

DOI

10.1017/S0033583516000081

More information

Latest update

9/5/2018 5