Aborting meiosis allows recombination in sterile diploid yeast hybrids
Journal article, 2021
Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.
Author
Simone Mozzachiodi
University of Côte d'Azur
Lorenzo Tattini
University of Côte d'Azur
Agnès Llored
University of Côte d'Azur
Agurtzane Irizar
University of Côte d'Azur
Neža Škofljanc
University of Côte d'Azur
Melania D’Angiolo
University of Côte d'Azur
Matteo De Chiara
University of Côte d'Azur
Benjamin P. Barré
University of Côte d'Azur
Jia Xing Yue
Sun Yat-Sen University
Angela Lutazi
University of Côte d'Azur
Sophie Loeillet
Institut Curie
Raphaelle Laureau
Columbia University
Souhir Marsit
Institut Curie
Simon Stenberg
Student at Chalmers
Benoit Albaud
Institut Curie
Karl Persson
University of Gothenburg
Jean-Luc Legras
University of Montpellier
Sylvie Dequin
University of Montpellier
Jonas Warringer
University of Gothenburg
Alain Nicolas
University of Côte d'Azur
Gianni Liti
University of Côte d'Azur
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 12 6564Subject Categories
Evolutionary Biology
Cell Biology
Microbiology
Genetics
DOI
10.1038/s41467-021-26883-8