An oomycete NLP cytolysin forms transient small pores in lipid membranes
Journal article, 2022
Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.
Author
Katja Pirc
National Institute of Chemistry
Luke A. Clifton
STFC Rutherford Appleton Laboratory
Neval Yilmaz
RIKEN
Andrea Saltalamacchia
Scuola Internazionale Superiore di Studi Avanzati
Mojca Mally
University of Ljubljana
Tina Snoj
National Institute of Chemistry
Nada Žnidaršič
University of Ljubljana
Marija Srnko
National Institute of Chemistry
Jure Borišek
National Institute of Chemistry
Petteri Parkkila
University of Helsinki
Chalmers, Physics, Nano and Biophysics
Isabell Albert
University of Erlangen-Nuremberg (FAU)
University of Tübingen
Marjetka Podobnik
National Institute of Chemistry
Keiji Numata
RIKEN
Thorsten Nürnberger
University of Johannesburg
University of Tübingen
Tapani Viitala
University of Helsinki
Jure Derganc
University of Ljubljana
Alessandra Magistrato
Scuola Internazionale Superiore di Studi Avanzati
Laboratorio Nazionale TASC
Jeremy H. Lakey
Newcastle University
Gregor Anderluh
National Institute of Chemistry
Science advances
2375-2548 (eISSN)
Vol. 8 10 eabj9406- eabj9406Subject Categories
Cell Biology
Biochemistry and Molecular Biology
Plant Biotechnology
DOI
10.1126/sciadv.abj9406
PubMed
35275729