An oomycete NLP cytolysin forms transient small pores in lipid membranes
Artikel i vetenskaplig tidskrift, 2022

Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.


Katja Pirc

Kemijski Inštitut

Luke A. Clifton

STFC Rutherford Appleton Laboratory

Neval Yilmaz


Andrea Saltalamacchia

Scuola Internazionale Superiore di Studi Avanzati

Mojca Mally

Univerza V Ljubljani

Tina Snoj

Kemijski Inštitut

Nada Žnidaršič

Univerza V Ljubljani

Marija Srnko

Kemijski Inštitut

Jure Borišek

Kemijski Inštitut

Petteri Parkkila

Helsingin Yliopisto

Chalmers, Fysik, Nano- och biofysik

Isabell Albert

Friedrich-Alexander-Universität Erlangen Nurnberg (FAU)

Universität Tübingen

Marjetka Podobnik

Kemijski Inštitut

Keiji Numata


Thorsten Nürnberger

University of Johannesburg

Universität Tübingen

Tapani Viitala

Helsingin Yliopisto

Jure Derganc

Univerza V Ljubljani

Alessandra Magistrato

Scuola Internazionale Superiore di Studi Avanzati

Laboratorio Nazionale TASC

Jeremy H. Lakey

Newcastle University

Gregor Anderluh

Kemijski Inštitut

Science advances

2375-2548 (eISSN)

Vol. 8 10 eabj9406- eabj9406



Biokemi och molekylärbiologi






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