SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model
Journal article, 2023
Healing large bone defects remains challenging in orthopedic surgery and is often associated with poor outcomes and complications. A major issue with bioengineered constructs is achieving a continuous interface between host bone and graft to enhance biological processes and mechanical stability. In this study, we have developed a new bioengineering strategy to produce oriented biocompatible 3D PLGA/aCaP nanocomposites with enhanced osseointegration. Decellularized scaffolds -containing only extracellular matrix- or scaffolds seeded with adipose-derived mesenchymal stromal cells were tested in a mouse model for critical size bone defects. In parallel to micro-CT analysis, SAXS tensor tomography and 2D scanning SAXS were employed to determine the 3D arrangement and nanostructure within the critical-sized bone. Both newly developed scaffold types, seeded with cells or decellularized, showed high osseointegration, higher bone quality, increased alignment of collagen fibers and optimal alignment and size of hydroxyapatite minerals.
SAXS tomography
PLGA/aCaP
Extracellular matrix
Critical size bone defect
Scaffold
Mesenchymal stromal cells
Author
Elisa A. Casanova
University Hospital of Zürich
Adrian Rodriguez Palomo
Chalmers, Physics, Materials Physics
Lisa Stähli
University Hospital of Zürich
Kevin Arnke
University Hospital of Zürich
Olivier Gröninger
Swiss Federal Institute of Technology in Zürich (ETH)
Melanie Generali
University of Zürich
Yvonne Neldner
University Hospital of Zürich
Simon Tiziani
University Hospital of Zürich
Ana Perez Dominguez
University of Zürich
Manuel Guizar-Sicairos
Paul Scherrer Institut
Zirui Gao
Paul Scherrer Institut
Christian Appel
Paul Scherrer Institut
Leonard Nielsen
Chalmers, Physics, Materials Physics
Marios Georgiadis
Stanford University
Franz E. Weber
University of Zürich
Wendelin Stark
Swiss Federal Institute of Technology in Zürich (ETH)
Hans Christoph Pape
University Hospital of Zürich
Paolo Cinelli
University of Zürich
University Hospital of Zürich
Marianne Liebi
Chalmers, Physics, Materials Physics
Swiss Federal Laboratories for Materials Science and Technology (Empa)
Biomaterials
0142-9612 (ISSN) 18785905 (eISSN)
Vol. 294 121989Subject Categories
Bio Materials
Biomaterials Science
Medical Materials
DOI
10.1016/j.biomaterials.2022.121989
PubMed
36628888