The Pseudomonas aeruginosa lectin LecB modulates intracellular reactive oxygen species production in human neutrophils
Journal article, 2024

Pseudomonas aeruginosa is a Gram-negative bacterium and an opportunistic pathogen ubiquitously present throughout nature. LecB, a fucose-, and mannose-binding lectin, is a prominent virulence factor of P. aeruginosa, which can be expressed on the bacterial surface but also be secreted. However, the LecB interaction with human immune cells remains to be characterized. Neutrophils comprise the first line of defense against infections and their production of reactive oxygen species (ROS) and release of extracellular traps (NETs) are critical antimicrobial mechanisms. When profiling the neutrophil glycome we found several glycoconjugates on granule and plasma membranes that could potentially act as LecB receptors. In line with this, we here show that soluble LecB can activate primed neutrophils to produce high levels of intracellular ROS (icROS), an effect that was inhibited by methyl fucoside. On the other hand, soluble LecB inhibits P. aeruginosa-induced icROS production. In support of that, during phagocytosis of wild-type and LecB-deficient P. aeruginosa, bacteria with LecB induced less icROS production as compared with bacteria lacking the lectin. Hence, LecB can either induce or inhibit icROS production in neutrophils depending on the circumstances, demonstrating a novel and potential role for LecB as an immunomodulator of neutrophil functional responses.

host-pathogen interaction


glycan-binding proteins




Felix Sanchez Klose

Chalmers, Life Sciences, Chemical Biology

Agnes Dahlstrand Rudin

University of Gothenburg

Linda Bergqvist

University of Gothenburg

Julia M. Scheffler

University of Gothenburg

Katarina Jönsson

University of Gothenburg

Ulrika Islander

University of Gothenburg

Anna Karlsson-Bengtsson

Chalmers, Life Sciences

University of Gothenburg

Johan Bylund

University of Gothenburg

Vignesh Venkatakrishnan

Chalmers, Life Sciences, Chemical Biology

European Journal of Immunology

0014-2980 (ISSN) 1521-4141 (eISSN)

Vol. 54 2 2350623

The glycome of emergency neutrophils and implications in sepsis

Swedish Research Council (VR) (2018-03077), 2019-01-01 -- 2024-12-31.

Subject Categories

Microbiology in the medical area





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