Bioimaging TOF-SIMS: localization of cholesterol in rat kidney sections.
Journal article, 2004

Here, we show the localization of a whole organic molecule in biological tissue using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Rat kidneys were sectioned by cryoultramicrotomy and dried at room temperature. The samples were covered with a thin silver layer and analyzed in an imaging TOF-SIMS instrument equipped with a Ga(+)-source. The cholesterol signal showed a high concentration in the nuclear areas of the epithelial cells and a lower concentration over areas representing the basal lamina of renal tubules. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells.

Cholesterol

Kidney

Computer-Assisted

Cryoultramicrotomy

chemistry

methods

Animals

Mass

chemistry

Rats

Image Processing

Spectrometry

Male

Microscopy

metabolism

ultrastructure

methods

Secondary Ion

metabolism

Rats

Silver

Sprague-Dawley

Author

Håkan Nygren

University of Gothenburg

Per Malmberg

University of Gothenburg

Christian Kriegeskotte

Heinrich F Arlinghaus

FEBS Letters

0014-5793 (ISSN)

Vol. 566 1-3 291-3

Subject Categories

MEDICAL AND HEALTH SCIENCES

DOI

10.1016/j.febslet.2004.04.052

PubMed

15147911