Dynamic microcompartmentalization of giant unilamellar vesicles by sol gel transition and temperature induced shrinking/swelling of poly(N-isopropyl acrylamide)
Journal article, 2007

Giant unilamellar vesicles (GUVs) were microinjected with aqueous solutions of poly(N-isopropyl acrylamide) (PNIPAAm). Temperature-dependent sol-gel phase transitions of the solutions, followed by shrinking and swelling of the resulting hydrogel, were studied in the presence of a variety of co-solutes within the GUV. Reversible formation of a dense, spherical hydrogel structure (compartment) was observed in all cases with defined shrinking/swelling behaviour at temperatures above the lower critical solution temperatures (LCSTs). Nanotube-mediated merging of two vesicles with thus formed compartments resulted in a single GUV with two internalized hydrogel structures. As an application example, we demonstrate how fluorescent nanoparticles can be immobilized in such gel structures.


Martin Markström

Chalmers, Chemical and Biological Engineering

Anders Gunnarsson


Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Aldo Jesorka

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Soft Matter

1744-683X (ISSN) 1744-6848 (eISSN)

Vol. 3 5 587-595

Subject Categories

Chemical Sciences



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