A chemical waveform synthesizer
Journal article, 2005

Algorithms and methods were developed to synthesize complex chemical waveforms in open volumes by using a scanning-probe microfluidic platform. Time-dependent variations and oscillations of one or several chemical species around the scanning probe, such as formation of sine waves, damped oscillations, and generation of more complex patterns, are demonstrated. Furthermore, we show that intricate bursting and chaotic calcium oscillations found in biological microdomains can be reproduced and that a biological cell can be used as a probe to study receptor functionalities as a function of exposure to time-dependent variations of receptor activators and inhibitors. Thus, the method allows for studies of biologically important oscillatory reactions. More generally, the system allows for detailed studies of complex time-varying chemical and physical phenomena in solution or at solution/surf ace interfaces. © 2005 by The National Academy of Sciences of the USA.

neurons

signal

pulses

receptors

cells

gradients

oscillations

solution exchange

channels

microfluidics

Author

Jessica Olofsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Helen Bridle

Chalmers

J. Sinclair

Chalmers

D. Granfeldt

Chalmers

Eskil Sahlin

University of Gothenburg

Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 102 23 8097-8102

Subject Categories

Social Sciences Interdisciplinary

DOI

10.1073/pnas.0500230102

More information

Latest update

9/10/2018