Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2008

A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. © 2008 Federation of European Biochemical Societies.

2-Methyl erythritol 4-phosphate

Isoprenoid

Mevalonate

Saccharomyces cerevisiae

Yeast

Författare

J. Maury

Danmarks Tekniske Universitet (DTU)

M. A. Asadollahi

Danmarks Tekniske Universitet (DTU)

K. Moller

CMC Biopharmaceuticals A/S

M. Schalk

Firmenich Inc.

A. Clark

Firmenich Inc.

L. R. Formenti

Danmarks Tekniske Universitet (DTU)

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

FEBS Letters

0014-5793 (ISSN)

Vol. 582 29 4032-4038

Ämneskategorier

Biokemi och molekylärbiologi

Annan industriell bioteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.febslet.2008.10.045