Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2008

A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. © 2008 Federation of European Biochemical Societies.


Saccharomyces cerevisiae


2-Methyl erythritol 4-phosphate



J. Maury

Danmarks Tekniske Universitet (DTU)

M. A. Asadollahi

Danmarks Tekniske Universitet (DTU)

K. Moller

CMC Biopharmaceuticals A/S

M. Schalk

Firmenich SA

A. Clark

Firmenich SA

L. R. Formenti

Danmarks Tekniske Universitet (DTU)

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

FEBS Letters

0014-5793 (ISSN)

Vol. 582 4032-4038


Biokemi och molekylärbiologi

Annan industriell bioteknik


Livsvetenskaper och teknik