Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing
Artikel i vetenskaplig tidskrift, 2008

Pucker up! A novel approach enabling direct measurements of biomolecular transfer across lipid bilayer membranes, using surface plasmon resonance, is demonstrated. The figure shows the transfer of sucrose (S) through melittin pores formed in surface-attached liposomes. By measuring the shift in refractive index in the volume enclosed by the liposomes, the sucrose transfer can be time-resolved and quantified.

CHOLESTEROL

melittin

LIPID VESICLES

MELITTIN

DNA

PLASMON RESONANCE SENSORS

SPR

liposomes

DRUG DISCOVERY

transport

surface plasmon resonance

membranes

Författare

Magnus Brändén

Chalmers, Teknisk fysik, Biologisk fysik

S. Dahlin

Stena Centre 1B

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

ChemPhysChem

1439-4235 (ISSN) 1439-7641 (eISSN)

Vol. 9 2480-2485

Ämneskategorier

Annan teknik

DOI

10.1002/cphc.200800614