Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation
Artikel i vetenskaplig tidskrift, 2005

This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix.

single cells

principal components analysis

surface-enhanced Raman scattering

chemical imaging

PCA

multivariate analysis

SERS

rhodamine 6G

lymphocyte

Författare

Charlotte Eliasson

Chalmers, Kemi- och bioteknik, Organisk och analytisk kemi

Anders Lorén

Chalmers, Kemi- och bioteknik, Organisk och analytisk kemi

Johan Engelbrektsson

Chalmers, Kemi- och bioteknik, Organisk och analytisk kemi

Mats Josefson

AstraZeneca AB

Jonas Abrahamsson

Göteborgs universitet

Katarina Abrahamsson

Chalmers, Kemi- och bioteknik, Organisk och analytisk kemi

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

1386-1425 (ISSN)

Vol. 61 4 755-760

Ämneskategorier

Analytisk kemi

Kemi

DOI

10.1016/j.saa.2004.05.038