Amelogenin is phagocytized and induces changes in integrin configuration, gene expression and proliferation of cultured human dermal fibroblasts
Artikel i vetenskaplig tidskrift, 2010

Fibroblasts are central in wound healing by expressing important mediators and producing and remodelling extracellular matrix (ECM) components. This study aimed at elucidating possible mechanisms of action of the ECM protein amelogenin on normal human dermal fibroblasts (NHDF). Amelogenin at 100 and 1000 μg/ml increased binding of NHDF via several integrins, including αvβ3, αvβ5 and α5β1. Further, both surface interaction and cellular uptake of amelogenin by NHDF was observed using scanning and transmission electron microscopy. Gene microarray studies showed >8-fold up or down-regulation of genes, of which most are involved in cellular growth, migration and differentiation. The effect of amelogenin was exemplified by increased proliferation over 7 days. In conclusion, the beneficial effects of amelogenin on wound healing are possibly conducted by stimulating fibroblast signalling, proliferation and migration via integrin interactions. It is hypothesized that amelogenin stimulates wound healing by providing connective tissue cells with a temporary extracellular matrix.

Författare

Sofia Almqvist

Göteborgs universitet

Maria Werthén

Göteborgs universitet

Anna Johansson

Göteborgs universitet

Peter Thomsen

Göteborgs universitet

Staale Petter Lyngstadaas

Journal of Materials Science: Materials in Medicine

0957-4530 (ISSN) 1573-4838 (eISSN)

Vol. 21 3 947-954

Ämneskategorier

Klinisk medicin

Biomaterialvetenskap

DOI

10.1007/s10856-009-3952-5