Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone.
Artikel i vetenskaplig tidskrift, 2003

Hormones released from the pituitary have been shown to regulate the expression of different proteins in the central nervous system. We wanted to examine whether peripheral administration of bovine growth hormone (bGH) regulates the expression of delta-opioid receptor (DOR) in the cerebral cortex and cerebellum. Expression of the DOR protein was quantified using Western blot densitometry. DOR mRNA was quantified with a solution hybridization RNase protection assay. Hypophysectomized (Hx) and untreated normal female rats were included in the study. All Hx rats were hormonally treated with cortisol (400 microg/kg/day) and L-thyroxine (10 microg/kg/day) for 19 days. Hypophysectomy resulted in a threefold increase in cerebral cortex and a twofold increase in cerebellum of the DOR protein compared with normal rats. One subgroup of Hx rats received bGH (1 mg/kg body weight) as a daily subcutaneous injection for 19 days. This treatment normalized the levels of DOR protein in the cerebral cortex and cerebellum. Immunohistochemical experiments showed that GH decreased DOR expression especially in layers II-VI in cerebral cortex and in stratum moleculare in cerebellum. Quantification of DOR mRNA by solution hybridization RNase protection assay corresponded to the DOR protein measurements. We conclude that the expression of DORs in cerebral cortex and cerebellum is regulated by GH.

pharmacology

Opioid

Receptors

Protein Biosynthesis

drug effects

Female

Sprague-Dawley

delta

metabolism

biosynthesis

Gene Expression Regulation

Rats

Cattle

drug effects

analysis

analysis

Cerebral Cortex

Messenger

Proteins

analysis

biosynthesis

chemistry

Cerebellum

Rats

chemistry

physiology

drug effects

metabolism

Growth Hormone

RNA

Animals

Författare

Anders I. Persson

Göteborgs universitet

N David Åberg

Göteborgs universitet

Jan Oscarsson

Göteborgs universitet

Olle Isaksson

Göteborgs universitet

Lars Rönnbäck

Göteborgs universitet

Fredrik Frick

Göteborgs universitet

Christian Sonesson

Göteborgs universitet

Chalmers, Institutionen för matematisk statistik

Peter S Eriksson

Göteborgs universitet

Journal of Neuroscience Research

0360-4012 (ISSN) 1097-4547 (eISSN)

Vol. 71 496-503

Ämneskategorier

Endokrinologi och diabetes

Neurologi

DOI

10.1002/jnr.10515

PubMed

12548705