Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone.
Artikel i vetenskaplig tidskrift, 2003
Hormones released from the pituitary have been shown to regulate the expression of different proteins in the central nervous system. We wanted to examine whether peripheral administration of bovine growth hormone (bGH) regulates the expression of delta-opioid receptor (DOR) in the cerebral cortex and cerebellum. Expression of the DOR protein was quantified using Western blot densitometry. DOR mRNA was quantified with a solution hybridization RNase protection assay. Hypophysectomized (Hx) and untreated normal female rats were included in the study. All Hx rats were hormonally treated with cortisol (400 microg/kg/day) and L-thyroxine (10 microg/kg/day) for 19 days. Hypophysectomy resulted in a threefold increase in cerebral cortex and a twofold increase in cerebellum of the DOR protein compared with normal rats. One subgroup of Hx rats received bGH (1 mg/kg body weight) as a daily subcutaneous injection for 19 days. This treatment normalized the levels of DOR protein in the cerebral cortex and cerebellum. Immunohistochemical experiments showed that GH decreased DOR expression especially in layers II-VI in cerebral cortex and in stratum moleculare in cerebellum. Quantification of DOR mRNA by solution hybridization RNase protection assay corresponded to the DOR protein measurements. We conclude that the expression of DORs in cerebral cortex and cerebellum is regulated by GH.
pharmacology
Opioid
Receptors
Protein Biosynthesis
drug effects
Female
Sprague-Dawley
delta
metabolism
biosynthesis
Gene Expression Regulation
Rats
Cattle
drug effects
analysis
analysis
Cerebral Cortex
Messenger
Proteins
analysis
biosynthesis
chemistry
Cerebellum
Rats
chemistry
physiology
drug effects
metabolism
Growth Hormone
RNA
Animals
Författare
Anders I. Persson
Göteborgs universitet
N David Åberg
Göteborgs universitet
Jan Oscarsson
Göteborgs universitet
Olle Isaksson
Göteborgs universitet
Lars Rönnbäck
Göteborgs universitet
Fredrik Frick
Göteborgs universitet
Christian Sonesson
Göteborgs universitet
Chalmers, Institutionen för matematisk statistik
Peter S Eriksson
Göteborgs universitet
Journal of Neuroscience Research
0360-4012 (ISSN) 1097-4547 (eISSN)
Vol. 71 4 496-503Ämneskategorier
Endokrinologi och diabetes
Neurologi
DOI
10.1002/jnr.10515
PubMed
12548705