Generation of focused electric field patterns at dielectric surfaces
Artikel i vetenskaplig tidskrift, 2005

We here report on a concept for creating well-defined electric field gradients between the boundaries of capillary electrode (a capillary of a nonconducting material equipped with an interior metal electrode) outlets, and dielectric surfaces. By keeping a capillary electrode opening close to a boundary between a conducting solution and a nonconducting medium, a high electric field can be created close to the interface by field focusing effects. By varying the inner and outer diameters of the capillary, the span of electric field strengths and the field gradient obtained can be controlled, and by varying the slit height between the capillary rim and the surface, or the applied current, the average field strength and gradient can be varied. Field focusing effects and generation of electric field patterns were analyzed using finite element method simulations. We experimentally verified the method by electroporation of a fluorescent dye (fluorescein diphosphate) into adherent, monolayered cells (PC-12 and WSS-1) and obtained a pattern of fluorescent cells corresponding to the focused electric field.

Författare

Jessica Olofsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

M. Levin

Cellectricon AB

A. Stromberg

Cellectricon AB

S. G. Weber

University of Pittsburgh

F. Ryttsen

Cellectricon AB

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 77 4667-4672

Ämneskategorier

Kemiteknik

DOI

10.1021/ac0502302