Protein adsorption on hydroxyapatite nanosensors with different crystal sizes studied in situ by a quartz crystal microbalance with the dissipation method
Artikel i vetenskaplig tidskrift, 2009

Hydroxyapatite (HAp) nanocrystals with different crystal sizes were deposited by the electrophoretic deposition method on the gold surface of a quartz crystal microbalance with a dissipation probe. The nanosensors formed this way were used to elucidate the adsorption mechanism of proteins with a similar pI value. The crystal sizes and the area of the a-plane affected only the adsorption amount of human serum albumin, but not that of bovine plasma fibrinogen. The viscoelastic property, Delta D/Delta f, of each absorbed layer on the nanosensors was almost constant. The protein adsorption mechanism can be explained as follows: the dissociated carboxyl groups (negative charge) of albumin were interacted with calcium ions and the hydrated amine groups (positive charge) at the alpha C domain of fibrinogen were with phosphate ions on the HAp surface.

spectroscopy

composite

vitro

serum

growth

ellipsometry

delivery

microparticles

liquid-chromatography

Surface-plasmon resonance

Författare

T. Ikoma

National Institute for Materials Science

M. Tagaya

Tokyo Institute of Technology

National Institute for Materials Science

N. Hanagata

National Institute for Materials Science

Tomohiko Yoshioka

Tokyo Institute of Technology

Dinko Chakarov

Chalmers, Teknisk fysik, Kemisk fysik

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

J. Tanaka

Tokyo Institute of Technology

Journal of the American Ceramic Society

0002-7820 (ISSN)

Vol. 92 5 1125-1128

Ämneskategorier

Atom- och molekylfysik och optik

DOI

10.1111/j.1551-2916.2009.02957.x

Mer information

Skapat

2017-10-07