Recombinant bacterial hemoglobin alters metabolism of Aspergillus niger
Artikel i vetenskaplig tidskrift, 2009

The filamentous fungus Aspergillus niger is used extensively for the production of enzymes and organic acids. A major problem in industrial fermentations with this fungus is to ensure sufficient supply of oxygen required for respiratory metabolism of the fungus. In case of oxygen limitation, the fungus will produce various by-products like organic acids and polyols. In order to circumvent this problem we here study the effects of the expression of a bacterial hemoglobin protein on the metabolism of A. niger. We integrated the vgb gene from Vitreoscilla sp. into the genome at the pyrA locus behind the strong gpdA promoter from Aspergillus nidulans. Analysis of secreted metabolites, oxygen uptake, CO2 evolution and biomass formation points towards a relief of stress in the mutant expressing VHB when it is exposed to oxygen limitation. Our findings therefore point to an interesting strategy to attenuate unwanted side effects resulting from oxygen limitation during industrial fermentations with A. niger. (C) 2008 Elsevier Inc. All rights reserved.

intracellular expression

nidulans

carbon

vitreoscilla hemoglobin

improves growth

gene

biotechnology

proteins

By-product formation

escherichia-coli

A. niger

Enzyme production

Metabolic engineering

enzyme

Författare

G. Hofmann

A. Diano

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 11 1 8-12

Ämneskategorier

Industriell bioteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.ymben.2008.07.002

Mer information

Skapat

2017-10-08