Nanotube-vesicle networks with functionalized membranes and interiors
Artikel i vetenskaplig tidskrift, 2003

We describe nanotube-vesicle networks with reconstituted membrane protein from cells and with interior activity defined by an injection of microparticles or molecular probes. The functionality of a membrane protein after reconstitution was verified by single-channel ion conductance measurements in excised inside-out patches from the vesicle membranes. The distribution of protein, determined by fluorescence detection, in the network membrane was homogeneous and could diffuse via a nanotube connecting two vesicles. We also show how injecting small unilamellar protein-containing vesicles can differentiate the contents of individual containers in a network. The combination of membrane activity and interior activity was demonstrated by ionophore-assisted accumulation, and internal Calcium Green-mediated detection, of Ca2+ within a single network container. This system can model a variety of biological functions and complex biological multicompartment structures and might serve as a platform for constructing complex sensor and computational devices.

Fluorescence

Biomimetic Materials/*chemistry

Microscopy

Signal Transduction

Organelles/chemistry/metabolism

Liposomes/*chemistry

Humans

Erythrocyte Membrane/chemistry/metabolism

Biosensing Techniques

Nanotechnology/*methods

Membrane Proteins/*chemistry/metabolism

Författare

M. Davidson

M. Karlsson

J. Sinclair

Kristin Sott

Göteborgs universitet

Owe Orwar

Institutionen för fysikalisk kemi

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 125 374-8

Ämneskategorier

Kemi