Oxygen radical production and severity of the Guillain--Barré syndrome.
Artikel i vetenskaplig tidskrift, 2007

The NADPH oxidase-dependent formation of reactive oxygen species ("oxygen radicals") by phagocytic cells constitutes an important part of the innate immune defence against microorganisms. Recent studies in animal models imply that a deficient function of the NADPH oxidase may be linked to the development of autoimmunity, but a link between oxygen radical production and severity of autoimmune disease in humans has not been established. We have examined the oxygen radical production in peripheral blood leukocytes from patients with the Guillain-Barré syndrome (GBS). Leukocytes from GBS patients in a stationary phase 1-5 years after their acute episode were activated by the formyl peptide receptor (FPR) ligand formyl-Met-Leu-Phe (fMLF) or the closely related formyl peptide like receptor 1 (FPRL1) ligand Trp-Lys-Tyr-Met-Val-Met-NH2 (WKYMVM). The patients were dichotomized according to severity by 1) the requirement of intensive care unit treatment and 2) the ability to walk independently after 3 months. Our data show that the amount of superoxide release following challenge with either of the two agonists fMLF and WKYMVM was significantly lower in patients requiring intensive care unit treatment or unable to walk after 3 months. Results obtained with the global activator phorbol myristate acetate, as well as with fMLF in TNF alpha-primed leukocytes, suggested that the deficiency of oxygen radical production in patients with severe GBS was the result of a specific deficiency of radical production in response to FPR/FPRL1 ligands rather than an inherent deficiency of NADPH oxidase function.

Severity

Leukocytes

NADPH Oxidase

Reactive Oxygen Species

Guillain-Barre Syndrome

Författare

Natalia Mossberg

Göteborgs universitet

Oluf Andersen

Göteborgs universitet

Staffan Nilsson

Chalmers, Matematiska vetenskaper

Göteborgs universitet

Claes Dahlgren

Göteborgs universitet

Kristoffer Hellstrand

Göteborgs universitet

Magnus Lindh

Göteborgs universitet

Åke Svedhem

Göteborgs universitet

Tomas Bergström

Göteborgs universitet

Charlotta Movitz

Göteborgs universitet

Journal of Neuroimmunology

0165-5728 (ISSN)

Vol. 192 1-2 186-91

Ämneskategorier

Mikrobiologi inom det medicinska området

DOI

10.1016/j.jneuroim.2007.09.020

PubMed

17945354