A Microfluidic Pipette for Single-Cell Pharmacology
Artikel i vetenskaplig tidskrift, 2010

We report on a free-standing microfluidic pipette made in poly(dimethylsiloxane) having a circulating liquid tip that generates a self-con-fining volume in front of the outlet channels. The method is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. The pipette is capable of carrying out a variety of complex fluid processing operations, such as mixing, multiplexing, or gradient generation at selected cells in cell and tissue cultures. Using an uptake assay, we show that it is possible to generate dose response curves in situ from adherent Chinese hamster ovary cells expressing proton-activated human transient receptor potential vanilloid (hTRPV1) receptors. Using confined superfusion and cell stimulation, we could activate hTRPV1 receptors in single cells, measure the response by a patch-clamp pipette, and induce membrane bleb formation by exposing selected groups of cells to formaldehyde/dithiothreitol-containing solutions, respectively. In short, the microfluidic pipette allows for complex, contamination-free multiple-compound delivery for pharmacological screening of intact adherent cells.

resolution

ion-channel

plasma-membrane

capsaicin receptor

stimulation

currents

Författare

Alar Ainla

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Erik Jansson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Natalia Stepanyants

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 82 11 4529-4536

Ämneskategorier

Kemi

DOI

10.1021/ac100480f