Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates
Artikel i vetenskaplig tidskrift, 2010

Supported lipid bilayers (SLBs) mimic biological membranes and are a versatile platform for a wide range of biophysical research fields including lipid-protein interactions, protein-protein interactions and membrane-based biosensors. the quartz crystal microbalance with dissipation monitoring (QCM-D) has had a pivotal role in understanding SLB formation on various substrates. as shown by its real-time kinetic monitoring of SLB formation, QCM-D can probe the dynamics of biomacromolecular interactions. We present a protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and discuss technical issues that need to be considered when working with charged lipid compositions. Furthermore, we explain a recently developed strategy that uses an amphipathic, a-helical (AH) peptide to form SLBs on gold and titanium oxide substrates. the protocols can be completed in less than 3 h.

c-virus ns5a

vesicle adsorption

protein adsorption

phosphatidyl serine

surface-plasmon resonance

size distribution



biomolecular adsorption



N. J. Cho

Stanford University

C. W. Frank

Stanford University

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Nature Protocols

1754-2189 (ISSN)

Vol. 5 1096-1106