Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates
Artikel i vetenskaplig tidskrift, 2010

Supported lipid bilayers (SLBs) mimic biological membranes and are a versatile platform for a wide range of biophysical research fields including lipid-protein interactions, protein-protein interactions and membrane-based biosensors. the quartz crystal microbalance with dissipation monitoring (QCM-D) has had a pivotal role in understanding SLB formation on various substrates. as shown by its real-time kinetic monitoring of SLB formation, QCM-D can probe the dynamics of biomacromolecular interactions. We present a protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and discuss technical issues that need to be considered when working with charged lipid compositions. Furthermore, we explain a recently developed strategy that uses an amphipathic, a-helical (AH) peptide to form SLBs on gold and titanium oxide substrates. the protocols can be completed in less than 3 h.

c-virus ns5a

vesicle adsorption

protein adsorption

phosphatidyl serine

surface-plasmon resonance

size distribution

in-situ

qcm-d

biomolecular adsorption

phospholipid-bilayers

Författare

N. J. Cho

Stanford University

C. W. Frank

Stanford University

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Nature Protocols

1754-2189 (ISSN)

Vol. 5 1096-1106

Ämneskategorier

Fysik

DOI

10.1038/nprot.2010.65