Single-cell electroporation
Artikel i vetenskaplig tidskrift, 2010
Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell. Unlike bulk electroporation, in which a homogenous electric field is applied to a suspension of cells, in SCEP an electric field is created locally near a single cell. Today, single-cell-level studies are at the frontier of biochemical research, and SCEP is a promising tool in such studies. In this review, we discuss pore formation based on theoretical and experimental approaches. Current SCEP techniques using microelectrodes, micropipettes, electrolyte-filled capillaries, and microfabricated devices are all thoroughly discussed for adherent and suspended cells. SCEP has been applied in in-vivo and in-vitro studies for delivery of cell-impermeant molecules such as drugs, DNA, and siRNA, and for morphological observations.
Transfection
patch-clamp
filled capillary
Cell membrane
Electric field
modeling electroporation
electric-field
Microelectrodes
genetic manipulation
mammalian-cells
Microfluidics
neurons in-vivo
membrane electropermeabilization
ellipsoidal cells
micro-electroporation
Författare
M. Y. Wang
University of Pittsburgh
Owe Orwar
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
Jessica Olofsson
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
S. G. Weber
University of Pittsburgh
Analytical and Bioanalytical Chemistry
1618-2642 (ISSN) 1618-2650 (eISSN)
Vol. 397 8 3235-3248Ämneskategorier
Kemi
DOI
10.1007/s00216-010-3744-2