Properties and Applications of Shear-Driven Lipid Bilayers
Doktorsavhandling, 2010

A supported lipid bilayer (SLB) is a lipid bilayer formed on a solid substrate. This system is currently one of the most common model systems used to imitate the properties of the cell membrane that surrounds human cells. In this work it was found that the hydrodynamic shear force from a liquid flow above an SLB can be used to move the entire SLB in the direction of the flowing liquid. This takes place in a rolling type of motion, with the lower monolayer of the SLB being essentially stationary on the support. Forming the SLB on the walls of a microfluidic channel provides a controlled and versatile method of handling and transporting the SLB and molecules associated with it. The theory behind, and some general observations, of this phenomenon are presented in this thesis. In addition of being able to drive the SLB over different types of surfaces and structures, this technique can also be used to manipulate the concentration of molecules solubilized in, or coupled to, the SLB. For example, it was found that membrane-bound proteins could be accumulated at the front of the advancing SLB, leading to a local increase in surface concentration of several orders of magnitude. This could allow the detection of membrane-associated molecules at much lower average surface coverage than previously possible. Another observation was that different types of membrane-associated molecules have a characteristic drift velocity in the lipid bilayer, under the same bulk flow in the channel. This opens up the possibility of separating membrane-associated molecules within the SLB based on differences in drift velocity, as in chromatography, but without the need of first extracting the molecules of interest from the lipid bilayer.


cell membrane


fluorescence microscopy



membrane proteins

lipid bilayer


model systems

FB-salen, Fysikgården 4, Chalmers tekniska högskola.
Opponent: Prof. Joachim Rädler, Department of Physics, Ludwig-Maximilians-University, Germany.


Peter Jönsson

Chalmers, Teknisk fysik, Biologisk fysik

Shear-Driven Motion of Supported Lipid Bilayers in Microfluidic Channels

Journal of the American Chemical Society,; Vol. 131(2009)p. 5294-5297

Artikel i vetenskaplig tidskrift

Effects of Surface Pressure and Internal Friction on the Dynamics of Shear-Driven Supported Lipid Bilayers

Langmuir,; Vol. 27(2011)p. 1430-1439

Artikel i vetenskaplig tidskrift

Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces

Analytical Chemistry,; Vol. 83(2011)p. 604-611

Artikel i vetenskaplig tidskrift

Mechanical Behavior of a Supported Lipid Bilayer under External Shear Forces

Langmuir,; Vol. 25(2009)p. 6279-6286

Artikel i vetenskaplig tidskrift

Sealing of Submicrometer Wells by a Shear-Driven Lipid Bilayer

Nano Letters,; Vol. 10(2010)p. 1900-1906

Artikel i vetenskaplig tidskrift





Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie: 3143

FB-salen, Fysikgården 4, Chalmers tekniska högskola.

Opponent: Prof. Joachim Rädler, Department of Physics, Ludwig-Maximilians-University, Germany.

Mer information