Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
Artikel i vetenskaplig tidskrift, 2010
The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals. With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques. Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.
Switzerland.
F-75700
UMR 5800
fermentative capacity
-8093 Zurich
quantitative-analysis
saccharomyces-cerevisiae strains
network
extraction
minimum information
glycolytic-enzymes
David] CNRS
metabolomics
chemostat cultures
genome
[Sherman
Lab Bordelais Rech Informat
Författare
A.B. Canelas
TU Delft
N. Harrison
University of Cambridge
A. Fazio
Danmarks Tekniske Universitet (DTU)
Jie Zhang
Chalmers, Kemi- och bioteknik, Livsvetenskaper
J. P. Pitkanen
Teknologian Tutkimuskeskus (VTT)
J. van den Brink
TU Delft
B.M. Bakker
Vrije Universiteit Amsterdam
L. Bogner
Universität Stuttgart
J. Bouwman
Vrije Universiteit Amsterdam
J. I. Castrillo
University of Cambridge
A. Cankorur
Bogazici Universitesi
Pramote Chumnanpuen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
P. Daran-Lapujade
TU Delft
D. Dikicioglu
Bogazici Universitesi
K. Van Eunen
Vrije Universiteit Amsterdam
J. C. Ewald
Eidgenössische Technische Hochschule Zürich (ETH)
J.J. Heijnen
TU Delft
B. Kirdar
Bogazici Universitesi
I. Mattila
Teknologian Tutkimuskeskus (VTT)
F.I. Mensonides
Vrije Universiteit Amsterdam
A. Niebel
Universität Stuttgart
M. Penttila
Teknologian Tutkimuskeskus (VTT)
J. Pronk
TU Delft
M. Reuss
Universität Stuttgart
L. Salusjarvi
Teknologian Tutkimuskeskus (VTT)
Uwe H. Sauer
Eidgenössische Technische Hochschule Zürich (ETH)
D. Sherman
Centre national de la recherche scientifique (CNRS)
M. Siemann-Herzberg
Universität Stuttgart
H.V. Westerhoff
Vrije Universiteit Amsterdam
J.H. de Winde
TU Delft
Dina Petranovic Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
S. G. Oliver
University of Cambridge
C. T. Workman
Danmarks Tekniske Universitet (DTU)
N. Zamboni
Eidgenössische Technische Hochschule Zürich (ETH)
Jens B Nielsen
Chalmers, Kemi- och bioteknik, Livsvetenskaper
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 1 9 145Styrkeområden
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Kemi
DOI
10.1038/ncomms1150