Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
Artikel i vetenskaplig tidskrift, 2010

The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals. With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques. Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.

metabolomics

Lab Bordelais Rech Informat

fermentative capacity

[Sherman

saccharomyces-cerevisiae strains

David] CNRS

quantitative-analysis

-8093 Zurich

glycolytic-enzymes

genome

network

minimum information

chemostat cultures

F-75700

Switzerland.

extraction

UMR 5800

Författare

A.B. Canelas

Technische Universiteit Delft

N. Harrison

University of Cambridge

A. Fazio

Danmarks Tekniske Universitet (DTU)

Jie Zhang

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

J. P. Pitkanen

Teknologian Tutkimuskeskus (VTT)

J. van den Brink

Technische Universiteit Delft

B.M. Bakker

VU University Amsterdam

L. Bogner

Universität Stuttgart

J. Bouwman

VU University Amsterdam

J. I. Castrillo

University of Cambridge

A. Cankorur

Bogazici Universitesi

Pramote Chumnanpuen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

P. Daran-Lapujade

Technische Universiteit Delft

D. Dikicioglu

Bogazici Universitesi

K. Van Eunen

VU University Amsterdam

J. C. Ewald

Eidgenössische Technische Hochschule Zürich (ETH)

J.J. Heijnen

Technische Universiteit Delft

B. Kirdar

Bogazici Universitesi

I. Mattila

Teknologian Tutkimuskeskus (VTT)

F.I. Mensonides

VU University Amsterdam

A. Niebel

Universität Stuttgart

M. Penttila

Teknologian Tutkimuskeskus (VTT)

J. Pronk

Technische Universiteit Delft

M. Reuss

Universität Stuttgart

L. Salusjarvi

Teknologian Tutkimuskeskus (VTT)

Uwe H. Sauer

Eidgenössische Technische Hochschule Zürich (ETH)

D. Sherman

CNRS Centre National de la Recherche Scientifique

M. Siemann-Herzberg

Universität Stuttgart

H.V. Westerhoff

VU University Amsterdam

J.H. de Winde

Technische Universiteit Delft

Dina Petranovic Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

S. G. Oliver

University of Cambridge

C. T. Workman

Danmarks Tekniske Universitet (DTU)

N. Zamboni

Eidgenössische Technische Hochschule Zürich (ETH)

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Nature Communications

2041-1723 (ISSN)

Vol. 1 9

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Kemi

DOI

10.1038/ncomms1150