Global estimation of mRNA stability in yeast
Artikel i vetenskaplig tidskrift, 2011

Turnover of mRNA is an important level of gene regulation. Individual mRNAs have different intrinsic stabilities. Moreover, mRNA stability changes dynamically with conditions such as hormonal stimulation or cellular stress. While accurate methods exist to measure the half-life of an individual transcript, global methods to estimate mRNA turnover have limitations in terms of resolution in time and precision. We describe and compare two complementary approaches to estimating global transcript stability: (1) direct measurement of decay rates; (2) indirect estimation of turnover from determination of mRNA synthesis rates and steady-state levels. Since the two approaches have distinct strengths yet confer different cellular perturbations, it is valuable to consider results obtained with both methods. The practical aspects of the chapter are written from a yeast perspective; the general considerations hold true for all eukaryotes, however.

Författare

Julia Marín-Navarro

Alexandra Jauhiainen

Göteborgs universitet

Chalmers, Matematiska vetenskaper

Joaquín Moreno

Paula Alepuz

José E. Pérez-Ortín

Per Sunnerhagen

Göteborgs universitet

Methods in molecular biology (Clifton, N.J.)

1064-3745 (ISSN)

Vol. 734 3-23

Ämneskategorier

Biokemi och molekylärbiologi

Annan biologi

DOI

10.1007/978-1-61779-086-7_1

Mer information

Skapat

2017-10-06