Heterologous production of polyunsaturated fatty acids in Saccharomyces cerevisiae causes a global transcriptional response resulting in reduced proteasomal activity and increased oxidative stress
Artikel i vetenskaplig tidskrift, 2011

Due to their health benefits there is much interest in developing microbial processes for efficient production of polyunsaturated fatty acids (PUFAs). In this study we co-expressed Mucor rouxii Delta(12)- and Delta(6)-desaturase genes in Saccharomyces cerevisiae, which resulted in a yeast strain that accumulated linoleic acid and gamma-linolenic acid in the different lipid species. Additionally, the strain contained higher levels of phospholipids and lower levels of ergosterol than the reference strain. Integrated analysis of the transcriptome revealed decreased expression of genes involved in ergosterol biosynthesis, but more unexpectedly it also pointed towards attenuated activity of the ubiquitin-proteasome system and a reduced oxidative stress response. In vitro and in vivo measurements showed reduced levels of all three proteasomal activities and also increased levels of reactive oxidative species in the PUFA-producing strain. Overall our results clearly show that PUFAs in yeast can be detrimental for several key cellular pathways, such as the oxidative stress response and proteasomal activity, suggesting that the membrane composition is of vital importance for these processes.

Desaturase

lipid-peroxidation

oxidized proteins

apoptosis

tolerance

degradation

PUFAs

yeast

yeast

membrane-lipids

disease

probe level

cross-linked protein

Stress response

Lipid biosynthesis

Författare

R. Ruenwai

King Mongkut's University of Technology Thonburi

A. Neiss

Chalmers, Kemi- och bioteknik

K. Laoteng

King Mongkut's University of Technology Thonburi

Wanwipa Vongsangnak

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

A. B. Dalfard

Shahid Bahonar University of Kerman

Chalmers, Kemi- och bioteknik

S. Cheevadhanarak

King Mongkut's University of Technology Thonburi

Dina Petranovic Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)

Vol. 6 3 343-356

Ämneskategorier

Industriell bioteknik

Styrkeområden

Livsvetenskaper och teknik (2010-2018)

DOI

10.1002/biot.201000316

Mer information

Senast uppdaterat

2019-07-17