A spectroscopic study of 2-[4′-(Dimethylamino)phenyl]-benzothiazole Binding to Insulin Amyloid Fibrils
Artikel i vetenskaplig tidskrift, 2010

The spectroscopic properties of 2-[4′-(dimethylamino)phenyl]- benzothiazole (BTA-2) in solution and in the presence of amyloid fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH3CN). BTA-2 binds to amyloid fibrils in solution leading to a characteristic blue-shift in the emission spectrum and an increase in fluorescence intensity. However, in solutions with increasing CH3CN concentration, there was a marked decrease in binding of the BTA-2 to fibrils. Studies demonstrating the effect of BTA-2 concentration on binding were performed. A comparison with the standard amyloid fluorescent marker, thioflavin T (ThT), showed that BTA-2 is more fluorescent, making it an excellent dye to label amyloid samples.

Thioflavin T

Fluorescence

BTA-2

2-[4′-(Dimethylamino)phenyl]-benzothiazole

Amyloid fibrils

Författare

Catherine Kitts

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

D. A. Vanden Bout

The University of Texas at Austin

Journal of Fluorescence

1053-0509 (ISSN) 1573-4994 (eISSN)

Vol. 20 4 881-889

Ämneskategorier

Fysikalisk kemi

DOI

10.1007/s10895-010-0634-0