Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations.
Artikel i vetenskaplig tidskrift, 2011

Single-cell gene expression levels show substantial variations among cells in seemingly homogenous populations. Astrocytes perform many control and regulatory functions in the central nervous system. In contrast to neurons, we have limited knowledge about functional diversity of astrocytes and its molecular basis. To study astrocyte heterogeneity and stem/progenitor cell properties of astrocytes, we used single-cell gene expression profiling in primary mouse astrocytes and dissociated mouse neurosphere cells. The transcript number variability for astrocytes showed lognormal features and revealed that cells in primary cultures to a large extent co-express markers of astrocytes and neural stem/progenitor cells. We show how subpopulations of cells can be identified at single-cell level using unsupervised algorithms and that gene correlations can be used to identify differences in activity of important transcriptional pathways. We identified two subpopulations of astrocytes with distinct gene expression profiles. One had an expression profile very similar to that of neurosphere cells, whereas the other showed characteristics of activated astrocytes in vivo.

Gene Expression Profiling

Mice

Gene Expression

metabolism

analysis

classification

methods

metabolism

Animals

Messenger

RNA

Stem Cells

Cells

Astrocytes

Cultured

Single-Cell Analysis

Up-Regulation

Författare

Anders Ståhlberg

Göteborgs universitet

Daniel Andersson

Göteborgs universitet

Johan Aurelius

Göteborgs universitet

Maryam Faiz

Göteborgs universitet

Marcela Pekna

Göteborgs universitet

Milos Pekny

Göteborgs universitet

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 39 4 e24-

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1093/nar/gkq1182

PubMed

21112872