Uptake of gold nanoparticles in healthy and tumor cells visualized by nonlinear optical microscopy
Artikel i vetenskaplig tidskrift, 2011

Understanding the mechanism underlying the interactions between inorganic nanostructures and biological systems is crucial for several rapidly growing fields that rely on nano-bio interactions. In particular, the further development of cell-targeted drug delivery using metallic nanoparticles (NP) requires new tools for understanding the mechanisms triggered by the contact of NPs with membranes in different cells at the subcellular level. Here we present a novel concept of multimodal microscopy, enabling three-dimensional imaging of the distribution of gold NPs in living, unlabeled cells. Our approach combines multiphoton induced luminescence (MIL) with coherent anti-Stokes Raman scattering (CARS) microscopy. Comparison with transmission electron microscopy (TEM) reveals in vivo sensitivity down to the single nanostructure. By monitoring the incorporation of NPs in human healthy epidermal keratinocytes and squamous carcinoma cells (SCC), we address the feasibility of noninvasive delivery of NPs for therapeutic purposes. While neutralizing PEG coating was confirmed to prevent NP integration in SCCs, an unexpectedly efficient integration of NPs into keratinocytes was observed. These results, independently validated using TEM, demonstrate the need for advanced surface modification protocols to obtain tumor selectivity for NP delivery. The CARS/MIL microscopy platform presented here is thus a promising tool for noninvasive study of the interaction between NPs and cell.

Carcinoma

Spectrum Analysis

Nonlinear Dynamics

Metal Nanoparticles

Electron

chemistry

chemistry

Humans

Transmission

Squamous Cell

Gold

Luminescence

Microscopy

cytology

Raman

Keratinocytes

pathology

Författare

G. Rago

FOM Institute for Atomic and Molecular Physics - AMOLF

Brigitte Bauer

Göteborgs universitet

Fredrik Svedberg

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Linda K Gunnarsson

Chalmers, Teknisk fysik, Bionanofotonik

Marica B Ericson

Göteborgs universitet

M. Bonn

FOM Institute for Atomic and Molecular Physics - AMOLF

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Journal of Physical Chemistry B

1520-6106 (ISSN) 1520-5207 (eISSN)

Vol. 115 5008-16

Ämneskategorier

Fysikalisk kemi

Atom- och molekylfysik och optik

DOI

10.1021/jp2009012

PubMed

21469683