Formation of pit-spanning phospholipid bilayers on nanostructured silicon dioxide surfaces for studying biological membrane events
Kapitel i bok, 2013

Zwitterionic phospholipid vesicles are known to adsorb and ultimately rupture on flat silicon dioxide (SiO 2 ) surfaces to form supported lipid bilayers. Surface topography, however, alters the kinetics and mechanistic details of vesicles adsorption, which under certain conditions may be exploited to form a suspended bilayer. Here we describe the use of nanostructured SiO 2 surfaces prepared by the colloidal lithography technique to scrutinize the formation of suspended 1-palmitoyl-2-oleoyl-sn-glycero-3- phosphocholine (POPC) lipid bilayers from a solution of small unilamellar lipid vesicles (SUV s ). Atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) were employed to characterize nanostructure fabrication and lipid bilayer assembly on the surface. © 2013 Springer Science+Business Media New York.

Författare

Indriati Pfeiffer

Erasmus University Medical Center

Michael Zäch

Chalmers, Teknisk fysik, Kemisk fysik

Methods in molecular biology (Clifton, N.J.)

1064-3745 (ISSN)

113-125

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Biokemi och molekylärbiologi

Atom- och molekylfysik och optik

DOI

10.1007/978-1-62703-336-7_12

ISBN

978-1-62703-335-0